Characterization and Biosynthesis of a Rare Fungal Hopane-Type Triterpenoid Glycoside Involved in the Antistress Property of Aspergillus fumigatus

被引：22
|
作者：
Ma, Ke [1 ,2 ]
Zhang, Peng [1 ]
Tao, Qiaoqiao [1 ]
Keller, Nancy P. [3 ]
Yang, Yanlong [4 ]
Yin, Wen-Bing [1 ,2 ]
Liu, Hongwei [1 ,2 ]
机构：
[1] Chinese Acad Sci, Inst Microbiol, State Key Lab Mycol, Beijing 100101, Peoples R China
[2] Univ Chinese Acad Sci, Savaid Med Sch, Beijing 100049, Peoples R China
[3] Univ Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA
[4] Lanzhou Univ, Coll Chem & Chem Engn, Lanzhou 730000, Gansu, Peoples R China
来源：
ORGANIC LETTERS | 2019年 / 21卷 / 09期
关键词：
D O I：
10.1021/acs.orglett.9b00984
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
A rare fungal hopane-type triterpenoid glycoside fumihopaside A was identified by genome mining in combination with HPLC-MS/MS in Aspergillus fumigatus. Combining genetic deletions in A. fumigatus with heterologous reconstitutions in Aspergillus nidulans of the afum gene cluster, we identified one fungal squalene hopane cyclase AfumA charging the cyclization of the hopene skeleton, one cytochrome P450, and one UDP-glycosyltransferase. Bioassays indicated that fumihopaside A plays important roles in protecting A. fumigatus against heat or ultraviolet stress.
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页码：3252 / 3256
页数：5
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