Gene expression profiling in glioblastoma and immunohistochemical evaluation of IGFBP-2 and CDC20

被引:62
|
作者
Marucci, Gianluca [2 ]
Morandi, Luca [2 ]
Magrini, Elisabetta [2 ]
Farnedi, Anna [2 ]
Franceschi, Enrico [3 ]
Miglio, Rossella [4 ]
Calo, Daniela [4 ]
Pession, Annalisa [2 ]
Foschini, Maria P. [2 ]
Eusebi, Vincenzo [1 ,2 ]
机构
[1] Osped Bellaria, Sez Anat Patol M Malpighi, I-40139 Bologna, Italy
[2] Univ Bologna, Sect Pathol, Bellaria Hosp, I-40139 Bologna, Italy
[3] Bellaria Hosp, Dept Med Oncol, Bologna, Italy
[4] Univ Bologna, Dept Stat Paolo Fortunati, Bologna, Italy
关键词
Glioblastoma; High-grade glioma; Gene expression; IGFBP-2; CDC20;
D O I
10.1007/s00428-008-0685-7
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Thirty-nine glial tumours (28 glioblastomas (GB) and 11 low-grade gliomas) were investigated with DNA microarrays to reveal a possible specific gene expression profile. Unsupervised classification through hierarchical cluster analysis identified two groups of tumours, the first composed of low-grade gliomas and the second mainly composed of GB. Nine genes were identified as most informative: seven were over-expressed in low-grade gliomas and under-expressed in GB; on the contrary, two genes, insulin-like growth factor binding protein 2 (IGFBP-2) and cell division cycle 20 homologue (CDC20), were over-expressed in GB and under-expressed in low-grade tumours. This same genetic profile was confirmed by reverse transcriptase polymerase chain reaction. Immunohistochemistry for IGFBP-2 was positive in 88.8% of the cases of GB and in only one low-grade glioma, whilst CDC20 immunostained 74.1% of the cases of GB and none low-grade glioma. This was confirmed in an additional series of cases studied with immunohistochemistry only. In conclusion, over-expression of mRNA levels of IGFBP-2 and CDC20 is highly related to GB, IGFBP-2 and CDC-20 gene and protein expressions are strongly correlated, and IGFBP-2 and CDC20 immunopositivity can be useful for the identification of GB in small biopsies.
引用
收藏
页码:599 / 609
页数:11
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