Preparation of Mono-disperse Bisphenol A Imprinted Polymer Microspheres and Their Chromatographic Properties

A facile method was presented for the synthesis of mono-disperse bisphenol A (BPA) imprinted polymer (MIP) microspheres. The polymerization procedure was similar to that of the conventional bulk polymerization except that a mixture of liquid paraffin and toluene was used as porogen. Results showed that the morphology, diameter, and the disperse degree of the particles were controlled by the ratio of toluene to liquid paraffin. When the ratio of toluene to liquid paraffin was 3 to 2 (VIV), the porogen was 5 mL, and ethylene glycol dimethacrylate (EGDMA) was about 24 mmol, monodisperse microspheres with diameters about 3 mu m could be synthesized. Chromatographic evaluation showed that the microspheres synthesized by this method had better imprinted properties compared to those of polymers synthesized by traditional bulk imprinted method. The method established may be an effective one for the preparation of MIP chromatographic stationary phases.