Diphenyl diselenide exerts anxiolytic-like effect in Wistar rats: Putative roles of GABAA and 5HT receptors

Diphenyl diselenide [(PhSe)(2)] is an organoselenium compound which presents pharmacological antioxidant, anti-inflammatory, antinociceptive and antidepressant properties. The present study was designed to investigate the anxiolytic effect of(PhSe)2 in rats, employing the elevated plus maze task. The involvement of 5HT and GABA receptors in the anxiolytic-like effect was also evaluated. (PhSe)(2) (5, 25 and 50 mu mol/kg. i.p.) did not affect locomotor activity as evaluated in the open open-field test, and learning and memory when assessed in the inhibitory foot-shock avoidance task. However, (PhSe)(2) at the 50 mu mol/kg dose produced signs of an anxiolytic action, namely a decreased number of fecal boli in the open-field arena and an increased time spent in as well as an increased number of entries to the open arms of the elevated plus maze test. To evaluate the role of GABA and 5HT receptors in the anxiolytic-like effect of (PhSe)(2), a selective GABA(A) receptor antagonist bicuculline, (0.75 mg/kg, i.p.), a non-selective 5HT(2A/2C) receptor antagonist, ritanserin (2 mg/kg, i.p.), a selective 5HT(2A/2C) receptor antagonist, ketanserin (1 mg/kg, i.p.), and a selective 5HT(1A) receptor antagonist, WAY100635 (0.1 mg/kg. i.p.) were used. All the antagonists used were able to abolish the anxiolytic effect of (PhSe)2 suggesting that GABAA and 5HT receptors may play a role in the pharmacological property of this selenocompound in the central nervous system. (C) 2008 Elsevier Inc. All rights reserved.