Disease Detection and Management via Single Nanopore-Based Sensors

A study was conducted to demonstrate disease detection and management through single nanopore-based sensors. The goal of the study was to mimic the general principle of analyte detection by channels and refine it with a simpler measurement design. Investigations revealed that the interaction of a single analyte and the channel pore caused relatively long-lived conductance changes. It was assumed that the analyte reduced the conductance, but it was conceivable that a charged analyte bound to the pore mouth and modulated the conductance up or down by a field effect. Increasing the analyte concentration was found to increase the frequency of analyte-induced conductance changes and the mean time the pore was occupied by the analyte. The analyte concentration was measured directly from the mean channel current, assuming that the reaction stoichiometry was 1:1.