Fabrication of 3D Microfluidic Chip integrating Concentration gradient design and Perfusion system for Embryo coculture with Stromal cells

被引：0

作者：

Chang, Pei-Yu ^{[1
]}

Chang, Kuo-Wei ^{[1
]}

Lin, Ciou-Wen ^{[1
]}

Shih, Po-Chen ^{[1
]}

Liu, Cheng-Hsien ^{[1
]}

Li, Chin-Jung ^{[2
]}

Tien, Chang-Hung ^{[2
]}

Huang, Hong-Yuan ^{[2
]}

Hsu, Wen-Syang ^{[3
]}

Yao, Da-Jeng ^{[4
]}

Fan, Shih-Kang ^{[5
]}

机构：

[1] Natl Tsing Hua Univ, Dept Power Mech Engn, Hsinchu, Taiwan

[2] Chang Gung Mem Hosp, Dept Obstet & Gynecol, Linkou, Taiwan

[3] Natl Chiao Tung Univ, Dept Mech Engn, Hsinchu, Taiwan

[4] Natl Tsing Hua Univ, Inst NanoEngn & MicroSyst, Hsinchu, Taiwan

[5] Natl Taiwan Univ, Dept Mech Engn, Taipei, Taiwan

来源：

6TH IEEE INTERNATIONAL CONFERENCE ON NANO/MOLECULAR MEDICINE AND ENGINEERING (IEEE-NANOMED 2012) | 2012年

关键词：

3D chip; Thin membrane; mimetic uterus; CULTURE; GENERATION; DEVICES;

D O I：

暂无

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Fabrication of 3D chip that enables cell culture under continuous flow perfusion and repeated in situ observation by light microscopy is described. Thin polydimethylsiloxane PDMS) membrane was etched to create an array of through-holes, and sandwiched between two PDMS channels to form an upper chamber and lower chamber. Thus we fabricated a 3D microfluidic chip which integrates the concentration gradient design and perfusion system for proposed embryo coculture with stromal cells.

引用

页码：32 / 35

页数：4

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