Histochemical demonstration of a monocarboxylate transporter in the mouse perineurium with special reference to GLUT1

Peripheral nerves express GLUT1 in both endoneurial blood vessels and the perineurium and utilize glucose as a major energy Substrate, as does the brain. However, under conditions of a reduced utilization of glucose., the brain is dependent upon monocarboxylates such as ketone bodies and lactate, being accompanied by an elevated expression of a monocarboxylate transporter (MCTI) in the blood-brain barrier. The present immunohistochemical study aimed to examine the expression or MCTI in the peripheral nerves of mice. MCTI immunoreactivity was found in the perineurial sheath and colocalized with GLUT1, while the endoneurial blood vessels expressed GLUT1 only. An intense expression of MCT1 in the perineurium was confirmed by Western blot and in situ hybridization analyses. Ultrastructurally, the MCTI and GLUT1 immunoreactivities in the thick perineurium showed an intensity gradient decreasing towards the innermost layer. In neonates, the MCT1 immunoreactivity in the perineurium was intense, while the GLUT1 immunoreactivity was faint or absent. These findings suggest that peripheral nerves depend oil rnonocarboxylates as a major energy source and that MCTI in the perineurium is responsible for the supply of rnonocarboxylates to nerve fibers and Schwann cells.