Studies on the affinity membrane for adsorption and purification of lysozyme (I) - Influence of low-temperature oxygen plasma treatment on surface structure and adsorption properties of the affinity membrane

In this paper, low-temperature oxygen plasma method was used to modify the surface of PP (polypropylene) microporous membranes, The influence of oxygen plasma on the chemical structure and physical performance of the membrane was investigated with SEM, XPS and IR spectra, The results of XPS and IR showed that polar groups, -OH, -COOH, C = O were created on these membrane surfaces. The groups -OH and -COOH were useful for activation and grafting process for affinity membrane with Cu2+ as a metal-chelating ligand, The influence of plasma treatment condition on the adsorbability of affinity membrane for lysozyme was carried out. The optimal adsorbability of lysozyme was 8 mu g/cm(2) on the affinity membrane prepared for 20 min and under 68 W plasma condition.