Bone morphogenetic protein-2 in biodegradable gelatin and β-tricalcium phosphate sponges enhances the in vivo bone-forming capability of bone marrow mesenchymal stem cells

被引:29
|
作者
Tadokoro, Mika [1 ]
Matsushima, Asako [1 ]
Kotobuki, Noriko [1 ]
Hirose, Motohiro [2 ]
Kimura, Yu [3 ]
Tabata, Yasuhiko [3 ]
Hattori, Koji [1 ]
Ohgushi, Hajime [1 ]
机构
[1] Natl Inst Adv Ind Sci & Technol, Hlth Res Inst, Amagasaki, Hyogo 6610974, Japan
[2] Natl Inst Adv Ind Sci & Technol, Human Technol Res Inst, Tsukuba, Ibaraki 3058566, Japan
[3] Kyoto Univ, Inst Frontier Med Sci, Dept Biomat, Field Tissue Engn,Sakyo Ku, Kyoto 6068507, Japan
关键词
gelatin; bone morphogenetic protein-2 (BMP-2); controlled drug release; mesenchymal stem cell; osteogenesis; OSTEOGENIC DIFFERENTIATION; HYDROXYAPATITE; VITRO; COMBINATION; TECHNOLOGY; CERAMICS; RHBMP-2; RELEASE; BMP-2;
D O I
10.1002/term.427
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Bone marrow mesenchymal stem cells (MSCs) have been used for bone tissue engineering due to their osteogenic differentiation capability, but their application is controversial. To enhance their capability, we prepared biodegradable gelatin sponges incorporating beta-tricalcium phosphate ceramics (GT sponge), which has been shown to possess excellent controlled drug-release properties. The GT sponge was used as a carrier for both rat MSCs and bone morphogenetic protein-2 (BMP-2) and osteogenic differentiation was assessed by subcutaneous implantation of four different kinds of implants, i.e. GT-alone, MSCGT composites, BMPGT composites and BMPGT composites supplemented with MSCs (BMPMSCGT) in rats. Two weeks after implantation, histological sections showed new bone formation in the peripheral parts of the BMPGT and in almost the total volume of the BMPMSCGT implants. After 4 weeks, histology as well as microCT analyses demonstrated extensive bone formation in BMPMSCGT implants. Gene expression and biochemical analyses of both alkaline phosphatase and bone-specific osteocalcin confirmed the histological findings. These results indicate that the combination of MSCs, GT and BMP synergistically enhances osteogenic capability and provides a rational basis for their clinical application in bone reconstruction. Copyright (c) 2011 John Wiley & Sons, Ltd.
引用
收藏
页码:253 / 260
页数:8
相关论文
共 50 条
  • [1] Effect of Biodegradable Gelatin β-Tri Calcium Phosphate Sponges Containing Mesenchymal Stem Cells and Bone Morphogenetic Protein-2 on Equine Bone Defect
    Tsuzuki, Nao
    Nakao, Shougo
    Seo, Jong-pil
    Yamada, Kazutaka
    Haneda, Shingo
    Furuoka, Hidefumi
    Tabata, Yasuhiko
    Sasaki, Naoki
    JOURNAL OF EQUINE VETERINARY SCIENCE, 2014, 34 (07) : 903 - 910
  • [2] Osteoinductivity of gelatin/β-tricalcium phosphate sponges loaded with different concentrations of mesenchymal stem cells and bone morphogenetic protein-2 in an equine bone defect model
    Seo, Jong-pil
    Tsuzuki, Nao
    Haneda, Shingo
    Yamada, Kazutaka
    Furuoka, Hidefumi
    Tabata, Yasuhiko
    Sasaki, Naoki
    VETERINARY RESEARCH COMMUNICATIONS, 2014, 38 (01) : 73 - 80
  • [3] Osteoinductivity of gelatin/β-tricalcium phosphate sponges loaded with different concentrations of mesenchymal stem cells and bone morphogenetic protein-2 in an equine bone defect model
    Jong-pil Seo
    Nao Tsuzuki
    Shingo Haneda
    Kazutaka Yamada
    Hidefumi Furuoka
    Yasuhiko Tabata
    Naoki Sasaki
    Veterinary Research Communications, 2014, 38 : 73 - 80
  • [4] Enhanced Regeneration of Critical Bone Defects Using a Biodegradable Gelatin Sponge and β-Tricalcium Phosphate with Bone Morphogenetic Protein-2
    Matsumoto, Goichi
    Omi, Yasushi
    Kubota, Eiro
    Ozono, Satoru
    Tsuzuki, Hideko
    Kinoshita, Yukihiko
    Yamamoto, Masaya
    Tabata, Yasuhiko
    JOURNAL OF BIOMATERIALS APPLICATIONS, 2009, 24 (04) : 327 - 342
  • [5] In vivo bone-forming capacity of human bone marrow-derived stromal cells is stimulated by recombinant human bone morphogenetic protein-2
    Hiroshi Yamagiwa
    Naoto Endo
    Kunihiko Tokunaga
    Tadashi Hayami
    Hiroshi Hatano
    Hideaki E. Takahashi
    Journal of Bone and Mineral Metabolism, 2001, 19 : 20 - 28
  • [6] In vivo bone-forming capacity of human bone marrow-derived stromal cells is stimulated by recombinant human bone morphogenetic protein-2
    Yamagiwa, H
    Endo, N
    Tokunaga, K
    Hayami, T
    Hatano, H
    Takahashi, HE
    JOURNAL OF BONE AND MINERAL METABOLISM, 2001, 19 (01) : 20 - 28
  • [7] Proliferation of equine bone marrow-derived mesenchymal stem cells in gelatin/β-tricalcium phosphate sponges
    Seo, Jong-pil
    Tsuzuki, Nao
    Haneda, Shingo
    Yamada, Kazutaka
    Furuoka, Hidefumi
    Tabata, Yasuhiko
    Sasaki, Naoki
    RESEARCH IN VETERINARY SCIENCE, 2012, 93 (03) : 1481 - 1486
  • [8] Effects of a synovial flap and gelatin/β-tricalcium phosphate sponges loaded with mesenchymal stem cells, bone morphogenetic protein-2, and platelet rich plasma on equine osteochondral defects
    Seo, Jong-pil
    Kambayashi, Yoshinori
    Itho, Megumi
    Haneda, Shingo
    Yamada, Kazutaka
    Furuoka, Hidefumi
    Tabata, Yasuhiko
    Sasaki, Naoki
    RESEARCH IN VETERINARY SCIENCE, 2015, 101 : 140 - 143
  • [9] Bone morphogenetic protein-2 enhances bone regeneration mediated by transplantation of osteogenically undifferentiated bone marrow-derived mesenchymal stem cells
    Kang, Sun-Woong
    La, Wan-Geun
    Kang, Jin Muk
    Park, Jung-Ho
    Kim, Byung-Soo
    BIOTECHNOLOGY LETTERS, 2008, 30 (07) : 1163 - 1168
  • [10] Bone morphogenetic protein-2 enhances bone regeneration mediated by transplantation of osteogenically undifferentiated bone marrow-derived mesenchymal stem cells
    Sun-Woong Kang
    Wan-Geun La
    Jin Muk Kang
    Jung-Ho Park
    Byung-Soo Kim
    Biotechnology Letters, 2008, 30 : 1163 - 1168