Dynamic regulation of uncoupling protein 2 content in INS-1E insulinoma cells

被引：37

作者：

Azzu, Vian ^{[1
]}

Affourtit, Charles ^{[1
]}

Breen, Eamon P. ^{[1
]}

Parker, Nadeene ^{[1
]}

Brand, Martin D. ^{[1
]}

机构：

[1] MRC, Dunn Human Nutr Unit, Cambridge CB2 0XY, England

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 2008年 / 1777卷 / 10期

基金：

英国医学研究理事会; 英国惠康基金;

关键词：

Glutamine; Half-life; Mitochondria; Pancreatic beta-cell; Serum starvation; UCP2;

D O I：

10.1016/j.bbabio.2008.07.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Uncoupling protein 2 (UCP2) regulates glucose-stimulated insulin secretion in pancreatic beta-cells. UCP2 content, measured by calibrated immunoblot in INS-1E insulinoma cells (a pancreatic beta-cell model) grown in RPMI medium, and INS-1E mitochondria, was 2.0 ng/million cells (7.9 ng/mg mitochondrial protein). UCP2 content was lower in cells incubated without glutamine and higher in cells incubated with 20 mM glucose, and varied from 1.0-4.4 ng/million cells (2.7-14.5 ng/mg mitochondrial protein). This dynamic response to nutrients was achieved by varied expression rates against a background of a very short UCP2 protein half-life of about 1 h. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：1378 / 1383

页数：6

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