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Distinct membrane compartmentalization and signaling of ephrin-A5 and ephrin-B1
被引:15
|作者:
Campbell, Tessa N.
[1
,2
]
Davy, Alice
[1
,2
,3
]
Liu, Yiping
[1
,2
]
Arcellana-Panlilio, Mayi
[1
,2
,4
]
Robbins, Stephen M.
[1
,2
]
机构:
[1] Univ Calgary, So Alberta Canc Res Inst, Dept Biochem & Mol Biol, Calgary, AB T2N 4N1, Canada
[2] Univ Calgary, So Alberta Canc Res Inst, Dept Oncol, Calgary, AB T2N 4N1, Canada
[3] Univ Toulouse, Ctr Dev Biol, Toulouse, France
[4] Univ Calgary, So Alberta Microarray Facil, Calgary, AB T2N 4N1, Canada
关键词:
lipid rafts;
detergent-resistant membrane;
ephrin-A5;
ephrin-B1;
compartmentalization;
differential signaling;
D O I:
10.1016/j.bbrc.2008.08.002
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
Eph receptor tyrosine kinases and their membrane-bound ligand ephrins form an essential cell communication system. Both ephrin classes have been shown to localize within cell surface lipid rafts, yet regulate different biological processes. In order to provide insight into this distinct behavior, we examined ephrin-A5 and B1 localization and signaling in murine fibroblasts and tissues. Results indicated that ephrin-A5 was constitutively present in detergent-resistant membrane fractions, while ephrin-B1 displayed translocation to membrane fractions upon stimulation. Ephrin-A5 and B1 were present in detergent-resistant membrane fractions with different buoyancies in vitro and in different raft fractions in vivo. Moreover, ephrin-A5 and B1 differentially influenced actin reorganization. Finally, microarray analysis revealed unique patterns of gene expression between the two ephrin classes. We thus demonstrate that distinct localization and compartmentalization provide insight into the subcellular basis for differential signaling observed in ephrin-A and B classes. (C) 2008 Elsevier Inc. All rights reserved.
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页码:362 / 366
页数:5
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