Production of xylitol and ethanol and activity of the key enzymes of D-xylose consumption in Pachysolen tannophilus mutant strains

被引：2
|
作者：
Bolotnikova, O. I. [1 ]
Trushnikova, E. P. [2 ]
Mikhailova, N. P. [2 ]
Ginak, A. I. [2 ]
机构：
[1] Petrozavodsk State Univ, Petrozavodsk, Russia
[2] Tech Univ, St Petersburg State Technol Inst, St Petersburg, Russia
来源：
MICROBIOLOGY | 2015年 / 84卷 / 04期
关键词：
D-xylose; xylitol; ethanol; xylose reductase; xylitol dehydrogenase; Pachysolen tannophilus; YEAST; DEHYDROGENASE; DEFICIENT; REDUCTASE;
D O I：
10.1134/S0026261715040049
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Production of xylitol and ethanol, as well as activities of the key enzymes of D-xylose consumption, were studied in Pachysolen tannophilus mutants with altered growth on D-xylose, xylitol, ethanol, or D-glucose as sole carbon sources. Suppressed activity of xylose reductase with preferential affinity for NADPH and of xylitol dehydrogenase to 4.40 and 4.80 mu mol mg(-1) min(-1), respectively, resulted in accumulation of xylitol (0.25 g per 1 g D-xylose consumed). The highest levels of NADH/NADPH-xylose reductase and xylitol dehydrogenase (6.00-6.80 and 6.80-8.40 mu mol mg(-1) min(-1), respectively) were found in the strains producing 0.24-0.26 g ethanol per 1 g D-xylose. Application of Pa. tannophilus mutants for analysis of the regulation of D-xylose catabolism in yeasts is discussed.
引用
收藏
页码：479 / 484
页数：6
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