Laser diffractometer for single-particle scattering measurements

被引：21
|
作者：
Ulanowski, Z [1 ]
Greenaway, RS [1 ]
Kaye, PH [1 ]
Ludlow, IK [1 ]
机构：
[1] Univ Hertfordshire, Dept Phys Sci, Hatfield AL10 9AB, Herts, England
来源：
MEASUREMENT SCIENCE AND TECHNOLOGY | 2002年 / 13卷 / 03期
关键词：
light scattering; single particle; laser diffractometry; optical trapping; levitation; refractive index; cell; spore; water content;
D O I：
10.1088/0957-0233/13/3/309
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
A laser diffractometer (polar nephelometer) constructed for single-particle studies is described. It takes advantage of an array of optical fibres and a scanning disc to allow the use of a single photomultiplier tube for the detection of scattered light. A background subtraction scheme makes the instrument especially valuable in cases where background scattering exceeds the scattering from the observed particle, for example for living cells in aqueous media. The system can be easily integrated with particle trapping equipment such as laser tweezers or an electrodynamic balance.
引用
收藏
页码：292 / 296
页数：5
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