Selective accumulation of heavy metals by three indigenous Bacillus strains, B-cereus, B-megaterium and B-sphaericus, from drain waters of a uranium waste pile

被引：44

作者：

Selenska-Pobell, S ^{[1
]}

Panak, P

Miteva, V

Boudakov, I

Bernhard, G

Nitsche, H

机构：

[1] Forschungszentrum Rossendorf, Inst Radiochem, D-01326 Dresden, Germany

[2] Bulgarian Acad Sci, Inst Microbiol, BU-1113 Sofia, Bulgaria

来源：

FEMS MICROBIOLOGY ECOLOGY | 1999年 / 29卷 / 01期

关键词：

amplified ribosomal DNA restriction analysis (ARDRA); bacillus; heavy metal bioaccumulation; inductive coupled plasma mass spectroscopy (ICP-MS); random amplified polymorphic DNA (RAPD);

D O I：

10.1016/S0168-6496(98)00130-5

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The interaction was studied of three Bacillus isolates, recovered from a uranium waste pile in Saxony, Germany, with different heavy metal ions present in the original ground water of the pile. Using 16S amplified ribosomal DNA restriction and random amplified polymorphic DNA analyses, the three isolates were classified as B. cereus, B. megaterium and B. sphaericus. The ability was investigated of vegetative cells and spores of the indigenous Bacillus strains as well as those of the corresponding reference strains B. cereus ATCC 4415, B. megaterium NRRL B5385, and B. sphaericus NCTC 9602 to accumulate Al, Ba, Cd, Co, Cr, Cs, Cu, Fe, Ga, Mn, Ni, Rb, Pb, Si, Sn, Sr, Ti, U, and Zn. It was clearly shown that all strains studied accumulated selectively large amounts of U, Pb, Cd, Cu and Al. Co, Mn, Ni, Zn, and Ga was only weakly accumulated. The binding of Pa, Ga, Mn, Ni, and Zn was species and in some cases even strain specific. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：59 / 67

页数：9