Investigation of calculating the FRET efficiency of donor-acceptor pair

被引：0

作者：

Wang, J ^{[1
]}

Sun, YH ^{[1
]}

Meng, JW ^{[1
]}

机构：

[1] Ocean Univ China, Opt & Optoelect Lab, Qingdao 266071, Peoples R China

来源：

SPECTROSCOPY AND SPECTRAL ANALYSIS | 2006年 / 26卷 / 02期

关键词：

emission spectrum of donor; excitation spectra of acceptor; efficiency of fluorescence resonance energy transfer (FRET); assessment method of product integral;

D O I：

暂无

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

The research on the method of calculating FRET efficiency is important to organic white LED research. In the present article, a new method of calculating the FRET efficiency of donor-acceptor pair by means of emission and excitation spectra was investigated. This method is easy to use to process data, needs only normal experiment equipments and doesn't need the parameters such as fluorescence quantum yield. However it can only be used to calculate comparative result. With this method, the FRET efficiency of fluorescein sodium, alpha-naphthol, bromocresol purple and riboflavin with different concentrations was studied, and the results match the experimental results completely, so this method has proved to be reasonable.

引用

页码：279 / 281

页数：3

共 7 条

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