Screening of protease-producing Serratia marcescens FS-3 and its application to deproteinization of crab shell waste for chitin extraction

被引：84
|
作者：
Jo, G. H. [1 ]
Jung, W. J. [1 ]
Kuk, J. H. [1 ]
Oh, K. T. [1 ]
Kim, Y. J. [1 ]
Park, R. D. [1 ]
机构：
[1] Chonnam Natl Univ, Inst Agr Sci & Technol, GSM NRL, Div Appl Biosci & Biotechnol, Kwangju 500757, South Korea
来源：
CARBOHYDRATE POLYMERS | 2008年 / 74卷 / 03期
关键词：
Serratia marcescens FS-3; crab shell; chitin extraction; Delvolase (R);
D O I：
10.1016/j.carbpol.2008.04.019
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Serratia marcescens FS-3, isolated from a soil sample in the southwestern area of Korea, exhibited a strong protease activity of 60.7 U/ml after 3 days of incubation. The protein removal from natural crab shell wastes with 10% S. marcescens FS-3 inoculum was 84% after 7 days of fermentation. At the same time, 47% demineralization occurred. When the shell waste was treated with 1% Delvolase(R) as a reference, deproteinization rate was 90%. In combination of 10% S. marcescens FS-3 culture supernatant and 1% Delvolase(R), deproteinization rate of the shell waste was 85%, while the rate was 81% in 10% FS-3 Culture Supernatant only. These results suggest that bio-deproteinization of the crab shell wastes using S. marcescens FS-3 Could be applicable to the chitin production process. (C) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码：504 / 508
页数：5
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