Enantioseparation of 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate-derivatized-amino acids by capillary zone electrophoresis using native and substituted beta-cyclodextrins as chiral additives .1. Discussion of optimum separation conditions

Enantioseparation of all 19 natural occurring chiral protein amino acids was investigated after derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) reagent by using capillary zone electrophoresis (CZE). Native and substituted beta-cyclodextrins were used as chiral selectors added to the background electrolyte: native beta-cyclodextrin, (2-hydroxy)propyl-beta-cyclodextrin, heptakis(2,6-di-O-methyl)-beta-cyclodextrin heptakis(2,3,6-tri-O-methyl)-beta-cyclodextrin, beta-cyclodextrin polymer and carboxymethyl-beta-cyclodextrin polymer. These selectors were compared with respect to enantioselectivity and analysis time, and the best suited selector specified for each amino acid. Optimum selector concentrations were experimentally determined for six of the amino acids.