A strategy for screening an inhibitor of viral silencing suppressors, which attenuates symptom development of plant viruses

被引：22

作者：

Shimura, Hanako ^{[1
]}

Fukagawa, Takako ^{[2
]}

Meguro, Ayano ^{[1
]}

Yamada, Hirokazu ^{[2
]}

Oh-hira, Mahito ^{[2
]}

Sano, Shinsuke ^{[2
]}

Masuta, Chikara ^{[1
]}

机构：

[1] Hokkaido Univ, Grad Sch Agr, Lab Cell Biol & Manipulat, Sapporo, Hokkaido 0608589, Japan

[2] Nippon Soda Co Ltd, Odawara Res Ctr, Kanagawa 2500280, Japan

来源：

FEBS LETTERS | 2008年 / 582卷 / 29期

关键词：

Double-stranded RNA; RNA silencing suppressor; Virus; Inhibitor;

D O I：

10.1016/j.febslet.2008.10.046

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To find out whether we can control plant virus diseases by blocking viral RNA silencing suppressors (RSSs), we developed a strategy to screen inhibitors that block the association of RSSs with siRNAs using a surface plasmon resonance assay. The screened chemicals were tested in competition with RSSs for binding to siRNAs using a mobility shift assay. We then confirmed that tested chemicals actually inhibited the RSS activity in vivo using a protoplast assay which was developed for this purpose. This entire system can be adapted to screening inhibitors of not only plant viruses but also some animal viruses possessing RSSs. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：4047 / 4052

页数：6

共 45 条

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Shimura, Hanako
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[4] Functional analyses of viral RNA silencing suppressors and a strategy to screen antiviral compounds that target viral RNA silencing suppressors
Hanako Shimura
Journal of General Plant Pathology, 2011, 77 : 361 - 363
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MOLECULAR PLANT-MICROBE INTERACTIONS, 2019, 32 (10) : 29 - 29
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PLANT JOURNAL, 2000, 22 (01): : 51 - 59
[7] Viral RNA silencing suppressors (RSS): Novel strategy of viruses to ablate the host RNA interference (RNAi) defense system
Bivalkar-Mehla, Shalmali
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VIRUS RESEARCH, 2011, 155 (01) : 1 - 9
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Ghosh, Dibyendu
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Chakraborty, Supriya
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2021, 105 (16-17) : 6301 - 6313
[9] Impact of viral silencing suppressors on plant viral synergism: a global agro-economic concern
Dibyendu Ghosh
Malavika M.
Supriya Chakraborty
Applied Microbiology and Biotechnology, 2021, 105 : 6301 - 6313
[10] Interaction between viral RNA silencing suppressors and host factors in plant immunity
Nakahara, Kenji S.
Masuta, Chikara
CURRENT OPINION IN PLANT BIOLOGY, 2014, 20 : 88 - 95

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