Camera array based light field microscopy

被引:127
|
作者
Lin, Xing [1 ,2 ]
Wu, Jiamin [1 ,2 ]
Zheng, Guoan [3 ]
Dai, Qionghai [1 ,2 ]
机构
[1] Tsinghua Univ, Dept Automat, Beijing 100084, Peoples R China
[2] Tsinghua Univ, Beijing Key Lab Multidimens & Multiscale Computat, Beijing 100084, Peoples R China
[3] Univ Connecticut, Biomed Engn & Elect Engn, Storrs, CT 06269 USA
来源
BIOMEDICAL OPTICS EXPRESS | 2015年 / 6卷 / 09期
关键词
Aperture plane - Data throughput - Frames per seconds - High resolution - Optical Metrology - Phase reconstruction - Prototype system - Spatial resolution;
D O I
10.1364/BOE.6.003179
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
This paper proposes a novel approach for high-resolution light field microscopy imaging by using a camera array. In this approach, we apply a two-stage relay system for expanding the aperture plane of the microscope into the size of an imaging lens array, and utilize a sensor array for acquiring different sub-apertures images formed by corresponding imaging lenses. By combining the rectified and synchronized images from 5 x 5 viewpoints with our prototype system, we successfully recovered color light field videos for various fast-moving microscopic specimens with a spatial resolution of 0.79 megapixels at 30 frames per second, corresponding to an unprecedented data throughput of 562.5 MB/s for light field microscopy. We also demonstrated the use of the reported platform for different applications, including post-capture refocusing, phase reconstruction, 3D imaging, and optical metrology. (C) 2015 Optical Society of America
引用
收藏
页码:3179 / 3189
页数:11
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