Comparative Enantioseparation of Crufomate with Cellulose- and Amylose-based Chiral Stationary Phases on Reverse-Phase and Normal-Phase High-Performance Liquid Chromatography

This study used cellulose- and amylose-based chiral stationary phases to comparatively separate crufomate enantiomers on reverse-phase and normal-phase high performance liquid chromatography (RP-HPLC and NP-HPLC). The baseline separations were obtained on Phenomene Lux cellulose-1, Lux cellulose-2 and Lux amylose-2 with 2-propanol/hexane and on Lux cellulose-1 and Lux amylose-2 with acetonitrile/water as mobile phase. The results of optical rotation detections showed that (+)-enantiomer of crufomate was firstly elated on Lux cellulose-1 and Lux amylose-2 both on NP-HPLC and RP-HPLC, while the (-)-enantiomer was firstly elated on Lux 1 cellulose-2 and Lux cellulose-3 on NP-HPLC. The influences of column temperature from 5-40 degrees C on separation were investigated on three chiral stationary phases. The thermodynamic parameters were calculated using van't Hoff equations to study the enthalpy-driven and entropy-driven process. This work will be helpful to prepare optical pure enantiomers and establish efficient analytical method for study, on enantioselective behaviours of crufomate.