Identification of Self-Incompatibility Alleles in Some Almond Genotypes by PCR Analysis

被引：0
|
作者：
Sharafi, Y. [1 ]
Mohammadi, S. A. [2 ]
Hajilou, J. [3 ]
Dejampour, J. [4 ]
机构：
[1] Islamic Azad Univ, Dept Hort, Maragheh Branch, Tabriz, Iran
[2] Islamic Azad Univ, Dept Agron & Plant Breeding, Tabriz, Iran
[3] Islamic Azad Univ, Dept Hort, Tabriz, Iran
[4] East Azarbaijan Agr & Nat Res Ctr, Tabriz, Iran
来源：
V INTERNATIONAL SYMPOSIUM ON PISTACHIOS AND ALMONDS | 2011年 / 912卷
关键词：
almond; S alleles; specific primers; self-incompatibly and inter-incompatibility; S-ALLELES; PRIMERS;
D O I：
10.17660/ActaHortic.2011.912.94
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Identification of S-alleles in almond cultivars and genotypes is important for breeders and growers. The aim of this study was to identify S allele patterns in 25 almond genotypes obtained from different geographical regions of East-Azarbaijan, Iran. Genomic DNA was extracted from young leaves and PCR was done using specific primers AmyC5R-AS1II, Alsc1-AmyC5R, Pruc2-Pruc4R and Pruc2-Pruc5R. Most of the S alleles were amplified using Pruc2-Pruc4R primer pair and the size of alleles ranged between 230-2000 bp in genotypes. All of the genotypes showed self-incompatibility pattern.
引用
收藏
页码：629 / 633
页数：5
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