Construction of recombinants Pseudomonas putida BO14 and Escherichia coli QEFCA8 for ferulic acid biotransformation to vanillin

被引：9

作者：

Okeke, BC ^{[1
]}

Venturi, V ^{[1
]}

机构：

[1] Int Ctr Genet Engn & Biotechnol, Bacteriol Unit, I-34012 Trieste, Italy

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 1999年 / 88卷 / 01期

关键词：

recombinants bacteria; biotransformation; ferulic acid; vanillin;

D O I：

10.1016/S1389-1723(99)80185-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Recombinants Pseudomonas putida BO14 and Escherichia coli QEFCA8 capable of ferulic acid biotransformation to vanillin mere constructed using homologous recombination and a PCR based cloning strategy, respectively. In the liquid culture of P. putida BO14, 26.81+/-2.30 mu g vanillin ml(-1) of culture filtrate was detected. In the case of recombinant E. coli QEFCA8, 19.37+/-1.95 mu g vanillin ml(-1) of culture filtrate was detected. Results indicate that the strains could be useful for the biotechnological production of vanillin, a very important flavoring substance.

引用

页码：103 / 106

页数：4