Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (ΔabcBA) Protects against Wild Type Brucella ovis in Rams

被引：16

作者：

Silva, Ana Patrcia C. ^{[1
]}

Macedo, Auricelio A. ^{[1
]}

Costa, Luciana F. ^{[2
]}

Rocha, Claudia E. ^{[2
]}

Garcia, Luize N. N. ^{[1
]}

Farias, Jade R. D. ^{[1
]}

Gomes, Priscilla P. R. ^{[1
]}

Teixeira, Gustavo C. ^{[1
]}

Fonseca, Kessler W. J. ^{[1
]}

Maia, Andrea R. F. ^{[1
]}

Neves, Gabriela G. ^{[1
]}

Romao, Everton L. ^{[1
]}

Silva, Teane M. A. ^{[1
]}

Mol, Juliana P. S. ^{[1
]}

Oliveira, Renata M. ^{[3
]}

Araujo, Marcio S. S. ^{[4
]}

Nascimento, Ernane F. ^{[1
]}

Martins-Filho, Olindo A. ^{[4
]}

Brandao, Humberto M. ^{[5
]}

Paixao, Tatiane A. ^{[2
]}

Santos, Renato L. ^{[1
]}

机构：

[1] Univ Fed Minas Gerais, Escola Vet, Dept Clin & Cirurgia Vet, BR-31270901 Belo Horizonte, MG, Brazil

[2] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Patol Geral, BR-31270901 Belo Horizonte, MG, Brazil

[3] Univ Estadual Maranhao, Dept Patol, BR-65057630 Sao Luis, MA, Brazil

[4] Fundacao Oswaldo Cruz, Ctr Pesquisas Rene Rachou, BR-30190002 Belo Horizonte, MG, Brazil

[5] Embrapa Gado Leite, BR-36038330 Juiz De Fora, MG, Brazil

来源：

PLOS ONE | 2015年 / 10卷 / 08期

关键词：

LIVE VACCINE; INFECTION; MICE; PCR; DIAGNOSIS; INSULIN;

D O I：

10.1371/journal.pone.0136865

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

This study aimed to evaluate protection induced by the vaccine candidate B. ovis Delta abcBA against experimental challenge with wild type B. ovis in rams. Rams were subcutaneously immunized with B. ovis Delta abcBA encapsulated with sterile alginate or with the non encapsulated vaccine strain. Serum, urine, and semen samples were collected during two months after immunization. The rams were then challenged with wild type B. ovis (ATCC25840), and the results were compared to non immunized and experimentally challenged rams. Immunization, particularly with encapsulated B. ovis Delta abcBA, prevented infection, secretion of wild type B. ovis in the semen and urine, shedding of neutrophils in the semen, and the development of clinical changes, gross and microscopic lesions induced by the wild type B. ovis reference strain. Collectively, our data indicates that the B. ovis Delta abcBA strain is an exceptionally good vaccine strain for preventing brucellosis caused by B. ovis infection in rams.

引用

页数：18

共 19 条

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