Strong expression of peroxisomal malate synthase genes from n-alkane-utilizable yeast Candida tropicalis in Saccharomyces cerevisiae

被引：1

作者：

Murai, T ^{[1
]}

Atomi, H ^{[1
]}

Ueda, M ^{[1
]}

Tanaka, A ^{[1
]}

机构：

[1] KYOTO UNIV,FAC ENGN,DEPT SYNTHET CHEM & BIOL CHEM,LAB APPL BIOL CHEM,SAKYO KU,KYOTO 60601,JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1996年 / 81卷 / 01期

关键词：

malate synthase; peroxisome; Candida tropicalis; Saccharomyces cerevisiae; promoter;

D O I：

10.1016/0922-338X(96)83121-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Expression of the genes encoding several peroxisomal enzymes of the n-alkane-utilizable yeast Candida tropicalis has been examined in Saccharomyces cerevisiae to find out a novel and foreign promoter functional in S. cerevisiae. Among these genes, two malate synthase genes, as web as the isocitrate lyase gene, were strongly expressed in acetate-grown cells of S. cerevisiae. The amounts of the recombinant malate synthases expressed in S. cerevisiae were as much as 30% of the total amount of soluble proteins in the cells. These results showed that the upstream regions (1.1 kbp for the MS-1 gene and 0.8 kbp for the MS-2 gene) of the malate synthase genes [UPR-MSs] from C. tropicalis contained strong promoters functional in S. cerevisiae and that the construction of novel gene expression systems-using UPR-MSs might be possible.

引用

页码：61 / 63

页数：3

共 40 条

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