Effects of Gold Nanoparticles on Endotoxin-Induced Uveitis in Rats

PURPOSE. This study evaluates the effects of the gold nanoparticle in endotoxin-induced uveitis in rats. METHODS. Adult male Wistar rats were divided into five groups: saline + saline, lipopolysaccharide (LPS) + saline, LPS + prednisolone, LPS + gold salt (GS) and LPS + gold nanoparticle (GNP). Two hours after LPS administration, prednisolone acetate 1%, GS, and GNP were topically applied to both eyes of rats and repeated every 6 hours for 24 hours. After 24 hours, rats were anesthetized and aqueous humor was sampled and the irides were removed. Aqueous humor TNF-alpha, myeloperoxidase activity were determined. Irides oxidative damage and content of toll-like receptor 4 (TLR4) and nuclear factor-kappa B (NF-kappa B) were determined. RESULTS. The administration of LPS-induced eye inflammatory response characterized by an increase in aqueous humor TNF-alpha, myeloperoxidase, and by irides oxidative damage. All these parameters were decreased by the administration of GNP. Since the inflammatory response secondary to LPS administration depends, in part, to the activation of the TLR4-NF-kappa B pathway we demonstrated here that a potential mechanism to explain the GNP effects was the decrease on TLR4 content and NF-beta B activation. CONCLUSIONS. These findings suggest that topical GNP decreases intraocular inflammation and oxidative damage by interfering in the TLR4-NF-kappa B pathway. (Invest Ophthalmol Vis Sci. 2012;53:8036-8041) DOI:10.1167/iovs.12-10743