Cellular effects of thirdhand tobacco smoke from smokers' homes

被引：3
|
作者：
Figueiro, Luciana Rizzieri [1 ]
Linden, Rafael [2 ]
Ziulkoski, Ana Luiza [3 ]
Mesquita Dantas, Denise Conceicao [4 ]
机构：
[1] Fed Univ Hlth Sci Porto Alegre UFCSPA, PPG Pathol, Sarmento Leite 245, BR-90050170 Porto Alegre, RS, Brazil
[2] Feevale Univ, Inst Hlth Sci, Toxicol Lab, Novo Hamburgo, Brazil
[3] Feevale Univ, Inst Hlth Sci, Cytotox Lab, Novo Hamburgo, Brazil
[4] UFCSPA, Pharmacosci Dept, Porto Alegre, RS, Brazil
来源：
TOXICOLOGY MECHANISMS AND METHODS | 2018年 / 28卷 / 04期
关键词：
Thirdhand tobacco smoke; environmental tobacco smoke; cytotoxicity; nicotine; tobacco; smoke; POLYCYCLIC AROMATIC-HYDROCARBONS; CYTO-TOXICITY ASSAYS; CIGARETTE-SMOKE; IN-VITRO; NEUTRAL RED; 3RD-HAND SMOKING; HUMAN SKIN; CYTOTOXICITY; CELLS; EXPOSURE;
D O I：
10.1080/15376516.2017.1411411
中图分类号：
R99 [毒物学（毒理学）];
学科分类号：
100405 ;
摘要：
After the cigarette is extinguished, many toxic compounds remain in the environment and accumulate in the air or on surfaces. This exposure is termed thirdhand smoke (THS) and its risks are poorly known. The aim of the study was to evaluate the cellular effects of THS from smokers' homes. Papers were placed in nine smoker's home and three nonsmoker's homes. An area equivalent to the paper size was cleaned with a cotton wipe. A549, Hep-2 and 3T3 cells were exposed to THS for 24h and cellular functions were assessed by MTT, neutral red (NR) reuptake and trypan blue exclusion assays. High levels of nicotine were found in samples from smokers' homes. Cellular proliferation was similar in almost all samples after THS exposure. Few changes in the cellular functions were observed, mainly higher mitochondrial activity, in paper samples. We are able to detect markers of THS collected from smokers' homes, but a clear evidence of cellular toxicity cannot be demonstrated by the present assays. This is the first study to evaluate the cellular effects of THS samples collected from smokers' homes.
引用
收藏
页码：243 / 251
页数：9
相关论文
共 50 条
  • [1] Thirdhand Tobacco Smoke: A Tobacco-Specific Lung Carcinogen on Surfaces in Smokers' Homes
    Thomas, Janet L.
    Hecht, Stephen S.
    Luo, Xianghua
    Ming, Xun
    Ahluwalia, Jasjit S.
    Carmella, Steven G.
    [J]. NICOTINE & TOBACCO RESEARCH, 2014, 16 (01) : 26 - 32
  • [2] Children's Exposure to Secondhand and Thirdhand Smoke Carcinogens and Toxicants in Homes of Hookah Smokers
    Kassem, Nada O. F.
    Daffa, Reem M.
    Liles, Sandy
    Jackson, Sheila R.
    Kassem, Noura O.
    Younis, Maram A.
    Mehta, Setoo
    Chen, Menglan
    Jacob, Peyton, III
    Carmella, Steve G.
    Chatfield, Dale A.
    Benowitz, Neal L.
    Matt, Georg E.
    Hecht, Stephen S.
    Hovell, Melbourne F.
    [J]. NICOTINE & TOBACCO RESEARCH, 2014, 16 (07) : 961 - 975
  • [3] Exposure to nitrosamines in thirdhand tobacco smoke increases cancer risk in non-smokers
    Ramirez, Noelia
    Oezel, Mustafa Z.
    Lewis, Alastair C.
    Marce, Rosa M.
    Borrull, Francesc
    Hamilton, Jacqueline F.
    [J]. ENVIRONMENT INTERNATIONAL, 2014, 71 : 139 - 147
  • [4] Metabolites of a Tobacco-Specific Lung Carcinogen in Children Exposed to Secondhand or Thirdhand Tobacco Smoke in Their Homes
    Thomas, Janet L.
    Guo, Hongfei
    Carmella, Steven G.
    Balbo, Silvia
    Han, Shaomei
    Davis, Andrew
    Yoder, Andrea
    Murphy, Sharon E.
    An, Larry C.
    Ahluwalia, Jasjit S.
    Hecht, Stephen S.
    [J]. CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION, 2011, 20 (06) : 1213 - 1221
  • [5] Wipe Sampling for Nicotine as a Marker of Thirdhand Tobacco Smoke Contamination on Surfaces in Homes, Cars, and Hotels
    Quintana, Penelope J. E.
    Matt, Georg E.
    Chatfield, Dale
    Zakarian, Joy M.
    Fortmann, Addie L.
    Hoh, Eunha
    [J]. NICOTINE & TOBACCO RESEARCH, 2013, 15 (09) : 1555 - 1563
  • [6] When smokers quit: exposure to nicotine and carcinogens persists from thirdhand smoke pollution
    Matt, Georg E.
    Quintana, Penelope J. E.
    Zakarian, Joy M.
    Hoh, Eunha
    Hovell, Melbourne F.
    Mahabee-Gittens, Melinda
    Watanabe, Kayo
    Datuin, Kathy
    Vue, Cher
    Chatfield, Dale A.
    [J]. TOBACCO CONTROL, 2017, 26 (05) : 548 - 556
  • [7] The effects of thirdhand smoke on reproductive health
    Liu, Huage
    Chen, Hao
    [J]. JOURNAL OF APPLIED TOXICOLOGY, 2022, 42 (02) : 172 - 179
  • [8] Thirdhand tobacco smoke: procedures to evaluate cytotoxicity in cell cultures
    Figueiro, Luciana Rizzieri
    Mesquita Dantas, Denise Conceicao
    Linden, Rafael
    Ziulkoski, Ana Luiza
    [J]. TOXICOLOGY MECHANISMS AND METHODS, 2016, 26 (05) : 355 - 361
  • [9] Thirdhand smoke and other challenges of tobacco control in the pediatric population
    Lidon-Moyano, Cristina
    Diez-Izquierdo, Ana
    Martinez-Sanchez, Jose M.
    [J]. ANALES DE PEDIATRIA, 2020, 93 (05): : 279 - 281
  • [10] Contribution of thirdhand smoke to overall tobacco smoke exposure in pediatric patients: study protocol
    Mahabee-Gittens, E. Melinda
    Matt, Georg E.
    Hoh, Eunha
    Quintana, Penelope J. E.
    Stone, Lara
    Geraci, Maegan A.
    Wullenweber, Chase A.
    Koutsounadis, Gena N.
    Ruwe, Abigail G.
    Meyers, Gabriel T.
    Zakrajsek, Mark A.
    Witry, John K.
    Merianos, Ashley L.
    [J]. BMC PUBLIC HEALTH, 2019, 19 (1)
12345