A method for measuring oxygen distributions in tissue using frequency domain phosphorometry

被引：34
|
作者：
Vinogradov, SA [1 ]
Fernandez-Seara, MA [1 ]
Dupan, BW [1 ]
Wilson, DF [1 ]
机构：
[1] Univ Penn, Sch Med, Dept Biochem & Biophys, Philadelphia, PA 19104 USA
来源：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY | 2002年 / 132卷 / 01期
关键词：
phosphorescence oxygen; maximum entropy method; lifetime distributions; luminescence quenching; oxygen distributions; heterogeneous objects; phosphorescence instrumentation;
D O I：
10.1016/S1095-6433(01)00541-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Phosphorescence lifetime of phosphors dissolved in biological fluids is dependent on the local oxygen concentration. When phosphor is added to the blood in vivo there is a distribution of lifetimes due to the distribution of oxygen concentrations in the blood of the sampled tissue volume. The distribution of oxygen can be assessed non-invasively and in practically real-time using frequency domain time-resolved phosphorometry. Here we describe a multi-frequency system for measurements of oxygen distributions in tissue in vivo. (C) 2002 Elsevier Science Inc. All rights reserved.
引用
收藏
页码：147 / 152
页数：6
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