SIMULTANEOUS DETERMINATION OF CHLORPHENIRAMINE MALEATE, PHENYLEPHRINE HYDROCHLORIDE, PARACETAMOL AND CAFFEINE IN PHARMACEUTICAL PREPARATION BY RP-HPLC

A reversed-phase High-Petformance Liquid-Chromatography (RP-HPLC) method was successfully developed for the simultaneous determination of quaternary mixture consisting of chlorpheniramine maleate (CPM), phenylephrine hydrochloride (PE), paracetamol (PCM) and caffeine in pharmaceutical preparation. The method was found to be simple, sensitive and rapid. The separation of the drugs was carried out using an Inertsil ODS C-18 column using 0.05M dibasic phosphate buffer: acetonitrile (93: 07; v/v) as the mobile phase. The flow rate of the mobile phase was adjusted to 1.5 ml/min and the column oven temperature was kept at 30 degrees C. All the drugs were resolved successfully with retention times 2.74 (CPM), 3.48 (PE), 9.5 (PCM) and 26.32 (caffeine) min when detection was carried out at 215 nm. The correlation coefficient was found to be 0.999, 0.998, 0.999 and 0.999, respectively for CPM, PE, PCM and caffeine. The relative standard deviation in the tablets was found to be less than 2% for sbc replicates. The method was validated for precision and accuracy. Thus, the proposed method can be successfully applicable to the pharmaceutical preparation containing the above mentioned drugs without any interference of excipients.