A Ternary Conjugation System for the Construction of DNA Libraries for Geobacillus kaustophilus HTA426

被引：13

作者：

Suzuki, Hirokazu ^{[1
]}

Wada, Keisuke ^{[2
]}

Furukawa, Megumi ^{[1
]}

Doi, Katsumi ^{[3
]}

Ohshima, Toshihisa ^{[3
]}

机构：

[1] Kyushu Univ, Grad Sch, Fac Agr, Fukuoka 8128581, Japan

[2] Kyushu Univ, Grad Sch, Fukuoka 8128581, Japan

[3] Kyushu Univ, Grad Sch, Fac Agr, Inst Genet Resources,Microbial Genet Div, Fukuoka 8128581, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2013年 / 77卷 / 11期

关键词：

Geobacillus kaustophilus; orotidine-5'-phosphate decarboxylase; pyrF; ternary conjugation; functional screening; DEEPEST SEA MUD; BACILLUS-THERMOCATENULATUS; SELECTION; GENE;

D O I：

10.1271/bbb.130492

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This report describes efficient plasmid uptake by the thermophile Geobacillus kaustophilus HTA426 by means of a ternary conjugation system, which was used to construct thermophile DNA libraries for G. kaustophilus and to identify the genes for orotidine-5'-phosphate decarboxylase by in vivo functional screening. The results indicate that the conjugation system is useful in constructing G. kaustophilus libraries, which are practical in identifying thermophile genes.

引用

页码：2316 / 2318

页数：3

共 21 条

[1] Counterselection System for Geobacillus kaustophilus HTA426 through Disruption of pyrF and pyrR
Suzuki, Hirokazu
Murakami, Ayano
Yoshida, Ken-ichi
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2012, 78 (20) : 7376 - 7383
[2] Frequent Transposition of Multiple Insertion Sequences in Geobacillus kaustophilus HTA426
Suzuki, Hirokazu
Taketani, Tatsunari
Tanabiki, Misaki
Ohara, Misaki
Kobayashi, Jyumpei
Ohshiro, Takashi
FRONTIERS IN MICROBIOLOGY, 2021, 12
[3] Genetic Transformation of Geobacillus kaustophilus HTA426 by Conjugative Transfer of Host-Mimicking Plasmids
Suzuki, Hirokazu
Yoshida, Ken-ichi
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2012, 22 (09) : 1279 - 1287
[4] A thiostrepton resistance gene and its mutants serve as selectable markers in Geobacillus kaustophilus HTA426
Wada, Keisuke
Kobayashi, Jyumpei
Furukawa, Megumi
Doi, Katsumi
Ohshiro, Takashi
Suzuki, Hirokazu
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2016, 80 (02) : 368 - 375
[5] Polysaccharide-Degrading Thermophiles Generated by Heterologous Gene Expression in Geobacillus kaustophilus HTA426
Suzuki, Hirokazu
Yoshida, Ken-ichi
Ohshima, Toshihisa
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2013, 79 (17) : 5151 - 5158
[6] Crystal structure of the n-terminal domain of Geobacillus kaustophilus HTA426 DnaD protein
Huang, Cheng-Yang
Chang, Yi-Wei
Chen, Wei-Ti
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2008, 375 (02) : 220 - 224
[7] Active Site Loop Conformation Regulates Promiscuous Activity in a Lactonase from Geobacillus kaustophilus HTA426
Zhang, Yu
An, Jiao
Yang, Guang-Yu
Bai, Aixi
Zheng, Baisong
Lou, Zhiyong
Wu, Geng
Ye, Wei
Chen, Hai-Feng
Feng, Yan
Manco, Giuseppe
PLOS ONE, 2015, 10 (02):
[8] Three inositol dehydrogenases involved in utilization and interconversion of inositol stereoisomers in a thermophile, Geobacillus kaustophilus HTA426
Yoshida, Ken-ichi
Sanbongi, Azusa
Murakami, Ayano
Suzuki, Hirokazu
Takenaka, Shinji
Takami, Hideto
MICROBIOLOGY-SGM, 2012, 158 : 1942 - 1952
[9] Characterization of a Novel Thermostable Carboxylesterase from Geobacillus kaustophilus HTA426 Shows the Existence of a New Carboxylesterase Family
Montoro-Garcia, Silvia
Martinez-Martinez, Irene
Navarro-Fernandez, Jose
Takami, Hideto
Garcia-Carmona, Francisco
Sanchez-Ferrer, Alvaro
JOURNAL OF BACTERIOLOGY, 2009, 191 (09) : 3076 - 3085
[10] Expression, purification and characterization of a novel NADH-dependent glutamate dehydrogenase from Geobacillus kaustophilus HTA426
Martinez, A. -B.
Zapata-Perez, R.
Hernandez-Garcia, S.
Garcia-Garcia, M. -I.
Perez-Gilabert, M.
Sanchez-Ferrer, A.
FEBS JOURNAL, 2013, 280 : 107 - 108

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