Orientational behavior of phospholipid membranes with mastoparan studied by 31P solid state NMR

被引：18
|
作者：
Hori, Y
Demura, M [1 ]
Niidome, T
Aoyagi, H
Asakura, T
机构：
[1] Hokkaido Univ, Grad Sch Sci, Div Biol Sci, Sapporo, Hokkaido 0600810, Japan
[2] Tokyo Univ Agr & Technol, Dept Biotechnol, Tokyo 1848588, Japan
[3] Nagasaki Univ, Fac Engn, Nagasaki 8528521, Japan
来源：
FEBS LETTERS | 1999年 / 455卷 / 03期
关键词：
mastoparan; nuclear magnetic resonance; membrane orientation; phospholipid; non-lamellar phase;
D O I：
10.1016/S0014-5793(99)00881-9
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Solid state P-31 NMR spectroscopy was used to study the perturbing effect of the wasp venom peptide mastoparan (MP) on lipid bilayers composed of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylglycerol (DMPG). The P-31 chemical shift anisotropy of multilamellar vesicles decreased with increasing peptide concentration, indicating that MP interacts strongly and selectively with the charged DMPG head group, Macroscopically oriented MP-lipid samples between glass plates were studied by P-31 NMR as a function of tilt angle. These spectra showed the coexistence of orientation-dependent lamellar signals as well as an isotropic peak, suggesting that MP can induce non-lamellar phases in DMPC/DMPG membranes. (C) 1999 Federation of European Biochemical Societies.
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收藏
页码：228 / 232
页数：5
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