A Sequence-tagged Site Marker for Identifying the Japanese Mat Rush (Juncus effusus) Cultivar 'Hinomidori'

被引：1
|
作者：
Noguchi, Tomotsugu [1 ,2 ,3 ]
Hosobuchi, Saeko [1 ,2 ]
Takamiya, Tomoko [1 ,2 ,8 ]
Iimure, Kazuhiko [4 ]
Saito, Akira [5 ]
Ohtake, Yuhko [6 ]
Yamashita, Hideji [7 ]
Murakami, Yasufumi [2 ]
Okuizumi, Hisato [1 ]
机构：
[1] Natl Agr & Food Res Org NARO, Genet Resources Ctr, Tsukuba, Ibaraki 3058602, Japan
[2] Tokyo Univ Sci, Fac Ind Sci Technol, Dept Biol Sci & Technol, Katsushika Ku, Tokyo 1258585, Japan
[3] Ind Technol Inst Ibaraki Prefecture, Ibaraki 3113195, Japan
[4] Kumamoto Prefectural Agr Res Ctr, Koshi, Kumamoto 8611113, Japan
[5] NARO, Kyushu Okinawa Agr Res Ctr, Agroenvironm Res Div, Koshi, Kumamoto 8611192, Japan
[6] NARO, Natl Agr & Food Res Org, Dept Cooperat & Communicat, Headquarter, Tsukuba, Ibaraki 3058517, Japan
[7] Tokai Univ, Sch Agr, Kumamoto 8691404, Japan
[8] Nihon Univ, Sch Pharm, Funabashi, Chiba 3058517, Japan
来源：
JARQ-JAPAN AGRICULTURAL RESEARCH QUARTERLY | 2017年 / 51卷 / 01期
关键词：
DNA polymorphism; PCR; RLGS; spot cloning; vegetatively propagated plant; RLGS METHOD; LANDMARK;
D O I：
10.6090/jarq.51.45
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
We have developed a genetic marker that can identify a registered variety of mat rush in Japan. A vegetatively propagated plant, mat rush is cultivated and used as the material for the surface layer of tatami mats in Japan. Because it has been difficult to detect DNA polymorphism among mat rush cultivars, we applied restriction landmark genome scanning (RLGS) to discriminate mat rush cultivars. RLGS is a genome analysis technique that can detect many DNA polymorphisms as spots separated by two-dimensional electrophoresis. By cloning the DNA of spots specific to the superior mat rush cultivar 'Hinomidori' detected by RLGS, we developed a sequence-tagged site (STS) marker for polymerase chain reaction (PCR) analyses. This STS marker makes it possible to distinguish 'Hinomidori' specifically from other mat rush cultivars. The strategy of developing the STS marker in this study is applicable to other vegetatively propagated plants that are characterized by difficult DNA polymorphism detection.
引用
收藏
页码：45 / 49
页数：5
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