Determination of sialic acids released from glycoproteins using capillary zone electrophoresis/electrospray ionization mass spectrometry

被引：20
|
作者：
Ortner, Karin [1 ]
Buchberger, Wolfgang [1 ]
机构：
[1] Johannes Kepler Univ Linz, Inst Analyt Chem, A-4040 Linz, Austria
来源：
ELECTROPHORESIS | 2008年 / 29卷 / 10期
关键词：
capillary zone electrophoresis-mass spectrometry; glycoproteins; N-acetylneuraminic acid; N-glycolyineuraminic acid; sialic acids;
D O I：
10.1002/elps.200700801
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A simple method for the determination of the two most abundant sialic acids released from glycoproteins based on CZE-MS is presented. Several parameters like BGE with various organic modifiers and sheath liquids were studied with respect to their suitability for the fast and easy analysis of the selected compounds by CZE-MS. Finally, a BGE containing 10 mM ammonium acetate allowed the quantification of N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc) in glycoproteins as well as human plasma samples. LOD and LOQ were 2 mu g/mL and 6 mu g/mL, respectively.
引用
收藏
页码：2233 / 2237
页数：5
相关论文
共 50 条
  • [1] CAPILLARY ZONE ELECTROPHORESIS MASS-SPECTROMETRY USING AN ELECTROSPRAY IONIZATION INTERFACE
    SMITH, RD
    OLIVARES, JA
    NGUYEN, NT
    UDSETH, HR
    ANALYTICAL CHEMISTRY, 1988, 60 (05) : 436 - 441
  • [2] Determination of isoflavones by capillary electrophoresis electrospray ionization mass spectrometry
    Aramendia, MA
    Borau, V
    Garcia, I
    Jimenez, C
    Lafont, F
    Marinas, JM
    Porras, A
    Urbano, FJ
    JOURNAL OF MASS SPECTROMETRY, 1995, : S153 - S157
  • [3] Determination of isoflavones by capillary electrophoresis/electrospray ionization mass spectrometry
    Aramendia, M.A.
    Borau, V.
    Garcia, I.
    Jimenez, C.
    Lafont, F.
    Marinas, M.
    Porras, A.
    Urbano, F.J.
    Journal of Mass Spectrometry, 1995, (Special):
  • [4] Sialic acid linkage differentiation of glycopeptides using capillary electrophoresis - electrospray ionization - mass spectrometry
    Kammeijer, Guinevere S. M.
    Jansen, Bas C.
    Kohler, Isabelle
    Heemskerk, Anthonius A. M.
    Mayboroda, Oleg A.
    Hensbergen, Paul J.
    Schappler, Julie
    Wuhrer, Manfred
    SCIENTIFIC REPORTS, 2017, 7
  • [5] Sialic acid linkage differentiation of glycopeptides using capillary electrophoresis – electrospray ionization – mass spectrometry
    Guinevere S. M. Kammeijer
    Bas C. Jansen
    Isabelle Kohler
    Anthonius A. M. Heemskerk
    Oleg A. Mayboroda
    Paul J. Hensbergen
    Julie Schappler
    Manfred Wuhrer
    Scientific Reports, 7
  • [6] Analysis of metal species by using electrospray ionization mass spectrometry and capillary electrophoresis electrospray ionization mass spectrometry
    Schramel, O
    Michalke, B
    Kettrup, A
    JOURNAL OF CHROMATOGRAPHY A, 1998, 819 (1-2) : 231 - 242
  • [7] Analysis of low-molecular-mass organic acids using capillary zone electrophoresis-electrospray ionization mass spectrometry
    Hagberg, J
    JOURNAL OF CHROMATOGRAPHY A, 2003, 988 (01) : 127 - 133
  • [8] IMPROVED ELECTROSPRAY IONIZATION INTERFACE FOR CAPILLARY ZONE ELECTROPHORESIS - MASS-SPECTROMETRY
    SMITH, RD
    BARINAGA, CJ
    UDSETH, HR
    ANALYTICAL CHEMISTRY, 1988, 60 (18) : 1948 - 1952
  • [9] Analysis of microcystins by capillary zone electrophoresis coupling with electrospray ionization mass spectrometry
    Tong, Ping
    Zhang, Lan
    He, Yu
    Tang, Shurong
    Cheng, Jintian
    Chen, Guonan
    TALANTA, 2010, 82 (04) : 1101 - 1106
  • [10] Analysis of metal species by using electrospray ionization mass spectrometry and capillary electrophoresis-electrospray ionization mass spectrometry
    J Chromatogr, 1-2 (231-242):
12345