Translation of small downstream ORFs enhances translation of canonical main open reading frames

被引：74

作者：

Wu, Qiushuang ^{[1
]}

Wright, Matthew ^{[1
]}

Gogol, Madelaine M. ^{[1
]}

Bradford, William D. ^{[1
]}

Zhang, Ning ^{[1
]}

Bazzini, Ariel A. ^{[1
,2
]}

机构：

[1] Stowers Inst Med Res, Kansas City, MO 64110 USA

[2] Univ Kansas, Med Ctr, Dept Mol & Integrat Physiol, Kansas City, KS 66103 USA

来源：

EMBO JOURNAL | 2020年 / 39卷 / 17期

关键词：

dORF; ribosome profiling; translation efficiency; MESSENGER-RNA DECAY; UPSTREAM ORFS; POLY(A) TAIL; IDENTIFICATION; CODONS;

D O I：

10.15252/embj.2020104763

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In addition to canonical open reading frames (ORFs), thousands of translated small ORFs (containing less than 100 codons) have been identified in untranslated mRNA regions (UTRs) across eukaryotes. Small ORFs in 5 ' UTRs (upstream (u)ORFs) often repress translation of the canonical ORF within the same mRNA. However, the function of translated small ORFs in the 3 ' UTRs (downstream (d)ORFs) is unknown. Contrary to uORFs, we find that translation of dORFs enhances translation of their corresponding canonical ORFs. This translation stimulatory effect of dORFs depends on the number of dORFs, but not the length or peptide they encode. We propose that dORFs represent a new, strong, and universal translation regulatory mechanism in vertebrates.

引用

页数：13

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