Light scattering from cells: finite-difference time-domain simulations and goniometric measurements

被引：251
|
作者：
Drezek, R
Dunn, A
Richards-Kortum, R
机构：
[1] Univ Texas, Biomed Engn Program, Austin, TX 78712 USA
[2] Univ Calif Irvine, Beckman Laser Inst & Med Clin, Irvine, CA 92697 USA
来源：
APPLIED OPTICS | 1999年 / 38卷 / 16期
关键词：
D O I：
10.1364/AO.38.003651
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
We have examined the Light-scattering properties of inhomogeneous biological cells through a combination of theoretical simulations and goniometric measurements. A finite-difference time-domain (FDTD) technique was used to compute intensity as a function of scattering angle for cells containing multiple organelles and spatially varying index of refraction profiles. An automated goniometer was constructed to measure the scattering properties of dilute cell suspensions. Measurements compared favorably with FDTD predictions. FDTD and experimental results indicate that scattering properties are strongly influenced by cellular biochemical and morphological structure. (C) 1999 Optical Society of America.
引用
收藏
页码：3651 / 3661
页数：11
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