Repeated elicitation enhances taxane production in suspension cultures of Taxus chinensis in bioreactors

被引：31
|
作者：
Wang, ZY [1 ]
Zhong, JJ [1 ]
机构：
[1] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
来源：
BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 06期
基金：
中国国家自然科学基金;
关键词：
elicitation; physiologically active substance; plant cell culture; taxoid production; Taxus chinensis;
D O I：
10.1023/A:1014549008516
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Methyl jasmonate added at 100 muM on days 8, 14 and 20 to cultures of Taxus chinensis growing in flasks and 1 l airlift bioreactors improved taxuyunnanine C (TC) production by 50-60% in flasks and by about 80% in airlift bioreactors compared with single addition of 100 muM methyl jasmonate on day 8. The final TC production in the airlift bioreactors reached 565+/-47 mg l(-1), which is the highest reported for taxoid production in a bioreactor.
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页码：445 / 448
页数：4
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