General misincorporation frequency: Re-evaluation of the fidelity of DNA polymerases

被引：6

作者：

Yang, Jie ^{[1
,2
]}

Li, Bianbian ^{[2
]}

Liu, Xiaoying ^{[2
]}

Tang, Hong ^{[1
]}

Zhuang, Xiyao ^{[1
]}

Yang, Mingqi ^{[2
]}

Xu, Ying ^{[2
]}

Zhang, Huidong ^{[2
]}

Yang, Chun ^{[1
]}

机构：

[1] Chongqing Med Univ, Pathogen Biol, Chongqing 400016, Peoples R China

[2] Sichuan Univ, West China Sch Publ Hlth, Chengdu 610041, Sichuan, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2018年 / 496卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Misincorporation frequency; DNA polymerase; Nucleotide incorporation; AERUGINOSA PHAGE PAP1; RIBONUCLEOTIDE INCORPORATION; CRYSTAL-STRUCTURE; KINETIC-ANALYSIS; CATALYTIC CORE; ERROR-PRONE; LESION-BYPASS; STERIC GATE; REPLICATION; ETA;

D O I：

10.1016/j.bbrc.2018.01.135

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA replication in cells is performed in the presence of four dNTPs and four rNTPs. In this study, we re-evaluated the fidelity of DNA polymerases using the general misincorporation frequency consisting of three incorrect dNTPs and four rNTPs but not using the traditional special misincorporation frequency with only the three incorrect dNTPs. We analyzed both the general and special misincorporation frequencies of nucleotide incorporation opposite dG, rG, or 8-oxoG by Pseudomonas aeruginosa phage 1 (PaP1) DNA polymerase Gp90 or Sulfolobus solfataricus DNA polymerase Dpo4. Both misincorporation frequencies of other DNA polymerases published were also summarized and analyzed. The general misincorporation frequency is obviously higher than the special misincorporation frequency for many DNA polymerases, indicating the real fidelity of a DNA polymerase should be evaluated using the general misincorporation frequency. (C) 2018 Elsevier Inc. All rights reserved.

引用

页码：1076 / 1081

页数：6

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