Development and application of a liquid chromatography/tandem mass spectrometric assay for measurement of N-acetylaspartate, N-acetylaspartylglutamate and glutamate in brain slice superfusates and tissue extracts

被引：20
|
作者：
Shah, Ajit J. [1 ]
de la Flor, Raul [1 ]
Atkins, Alan [1 ]
Slone-Murphy, Julia [1 ]
Dawson, Lee A. [1 ]
机构：
[1] GlaxoSmithKline Plc, Neurosci Ctr Excellence Drug Discovery, Harlow CM19 5AW, Essex, England
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2008年 / 876卷 / 02期
关键词：
N-Acetylaspartic acid; N-Acetylaspartylglutamic acid; Glutamic acid; Liquid chromatography-tandem mass spectrometry; Superfusion; Ex vivo;
D O I：
10.1016/j.jchromb.2008.10.012
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A liquid chromatography-tandem mass spectrometric method has been developed for measurement of N-acetylaspartate, N-acetylaspartylglutamate and glutamate. The analytes were separated within 5 min using an anion exchange/reverse phase column. The lower limit of quantification for Glu, NAA and NAAG was found to be 5, 50 and 6 nM, respectively, with a signal-to-noise ratio of 5:1. Using this methodology the basal levels of Glu, NAA and NAAG could be measured consistently in in vitro superfusion samples from rat hippocampus. The assay was also used for measurement of the distribution of Glu. NAA and NAAG in different regions of the rat brain. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码：153 / 158
页数：6
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