Modified mRNA-LNP Vaccines Confer Protection against Experimental DENV-2 Infection in Mice

被引：38
|
作者：
Zhang, Mengling [1 ,2 ,3 ]
Sun, Jin [1 ]
Li, Min [1 ]
Jin, Xia [3 ]
机构：
[1] Chinese Acad Sci, Inst Pasteur Shanghai, Viral Dis & Vaccine Translat Res Unit, CAS Key Lab Mol Virol & Immunol, Shanghai, Peoples R China
[2] Univ Chinese Acad Sci, Beijing, Peoples R China
[3] Fudan Univ, Shanghai Publ Hlth Clin Ctr, Shanghai, Peoples R China
来源：
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT | 2020年 / 18卷
基金：
美国国家科学基金会; 国家重点研发计划;
关键词：
TETRAVALENT DENGUE VACCINE; NONSTRUCTURAL PROTEIN NS1; DNA VACCINE; VIRUS; EFFICACY; IMMUNITY; ENHANCEMENT; ANTIBODIES; INFLUENZA; RESPONSES;
D O I：
10.1016/j.omtm.2020.07.013
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Dengue virus (DENV) infection is a major global public health concern, and there is no effective vaccine for it. In this study, we describe the design and characterization of three nucleotide-modified mRNA vaccines (prME-mRNA, E80-mRNA, and NS1-mRNA) for DENV-2. Our results showed that vaccination with E80-mRNA alone or a combination of E80-mRNA and NS1-mRNA can induce high levels of neutralizing antibodies and antigen-specific T cell responses; furthermore, these vaccines confer complete protection against DENV-2 challenge in immunocompetent mice. These data provide foundations for further development of a tetravalent DENV vaccine based on nucleotide-modified mRNA.
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页码：702 / 712
页数：11
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