Downregulation of Thy1 in retinal ganglion cells in experimental glaucoma

被引:97
|
作者
Huang, W [1 ]
Fileta, J [1 ]
Guo, Y [1 ]
Grosskreutz, CL [1 ]
机构
[1] Harvard Univ, Massachusetts Eye & Ear Infirm, Sch Med, Howe Lab Ophthalmol, Boston, MA 02114 USA
关键词
experimental glaucoma; laser capture microdissection; qRCR; Thy1; mRNA;
D O I
10.1080/02713680500545671
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
Purpose: Thy1 is a surface glycoprotein uniquely expressed in retinal ganglion cells (RGCs) in retina. The aim of this study was to investigate the expression change of Thy1 in a model of experimental glaucoma. Methods: The change of protein and mRNA levels of Thy1 in the retina were studied using stereological counts of back-labeled RGCs, Western blot analysis, immunohistochemistry, and laser capture microdissection (LCM) of RGCs with quantitative PCR analysis of mRNA in a model of experimental glaucoma. LCM after optic nerve crush was also performed to evaluate Thy1 mRNA levels after a different injury. Results: After 10 days of elevated IOP, there was a 34% loss of RGC number, Thy1 protein decreased 60% in eyes with elevated intraocular pressure (IOP), and Thy1 mRNA levels decreased 51% in RGCs. Both protein and mRNA level change of Thy1 is to a much greater extent than RGC number loss. Conclusions: The current results confirm that Thy 1 mRNA levels do not reflect the number of RGCs present and extend this to include a parallel decrease in Thy1 protein levels. These results suggest that Thy1 serves as an early marker of RGC stress, but not a marker of RGC loss, in models of retinal damage.
引用
收藏
页码:265 / 271
页数:7
相关论文
共 50 条
  • [1] Changes in Thy1 gene expression associated with damaged retinal ganglion cells
    Schlamp, CL
    Johnson, EC
    Li, Y
    Morrison, JC
    Nickells, RW
    MOLECULAR VISION, 2001, 7 (27): : 192 - 201
  • [2] Paeoniflorin Protects Retinal Ganglion Cells in Experimental Glaucoma
    Wu, Lina
    Chen, Zhen
    CURRENT TOPICS IN NUTRACEUTICAL RESEARCH, 2023, 21 (01) : 86 - 93
  • [3] Calpain is Activated in Retinal Ganglion Cells in Experimental Glaucoma
    Qu, J.
    Huang, W.
    Grosskreutz, C. L.
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2010, 51 (13)
  • [4] THY1(+) AND THY1(-) FIBROBLASTS RESPOND DIFFERENTLY TO RELEVANT MITOGENS
    MCINTOSH, JC
    RICHARDSON, TL
    AMERICAN REVIEW OF RESPIRATORY DISEASE, 1993, 147 (04): : A156 - A156
  • [5] COCULTURE OF BAL CELLS WITH THY1 (+) AND (-) FIBROBLASTS
    MCINTOSH, JC
    SUMMERS, WS
    RICHARDSON, TL
    CLINICAL RESEARCH, 1992, 40 (04): : A853 - A853
  • [6] Protection of ganglion cells in experimental glaucoma by retinal laser photocoagulation
    Nork, TM
    Poulsen, GL
    Nickells, RW
    Hoeve, JNV
    Cho, NC
    Levin, LA
    Lucarelli, MJ
    ARCHIVES OF OPHTHALMOLOGY, 2000, 118 (09) : 1242 - 1250
  • [7] Overexpression of Parkin Protects Retinal Ganglion Cells in Experimental Glaucoma
    Hu Xinxin
    Dai, Yi
    Sun, Xing-Huai
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2018, 59 (09)
  • [8] Effect of Doxycycline on Retinal Ganglion Cells in an Experimental Glaucoma Model
    Lee, Tae-eun
    Kim, Yong Yeon
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2017, 58 (08)
  • [9] Retinal ganglion cells adapt to ionic stress in experimental glaucoma
    Boal, Andrew M.
    McGrady, Nolan R.
    Holden, Joseph M.
    Risner, Michael L.
    Calkins, David J.
    FRONTIERS IN NEUROSCIENCE, 2023, 17
  • [10] Overexpression of parkin protects retinal ganglion cells in experimental glaucoma
    Dai, Yi
    Hu, Xinxin
    Sun, Xinghuai
    CELL DEATH & DISEASE, 2018, 9