Melaminivora jejuensis sp nov., isolated from Swinery waste

A sulfur-oxidizing bacterium, designated strain KBB12(T), was isolated from swinery waste collected in Jeju, Republic of Korea. The cells were Gram-stain-negative, flagellated and rod-shaped. Growth occurred at 15-45 degrees C (optimum, 30-37 degrees C), at pH 6-9 (optimum, pH 7.0) and in the presence of 0-1% (w/v) NaCl. The major cellular fatty acids were summed feature 3 (iso-C-15 : 0 2-OH and/or C-16 : 1 omega 7c, C-16 : 0 and C-18 : 1 omega 7c. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phospholipid and an unidentified lipid. The major isoprenoid quinone was ubiquinone-8 (Q-8) and the DNA G+C content of the genomic DNA was 69.6 mol%. Phylogenetic analyses, based on 16S rRNA gene sequences, showed that the novel isolate belongs to the genus Melaminivora and was most closely related to Melaminivora alkalimesophila CY1(T) (97.2% similarity). The DNA-DNA relatedness values between strain KBB12(T) and M. alkalimesophila DSM26005(T) was 43.4 +/- 2.7 %. On the basis of phylogenetic and phenotypic evidence, it is proposed that strain KBB12(T) represents a novel species of the genus Melaminivora, for which the name Melaminivora jejuensis sp. nov. is proposed. The type strain is KBB12(T) (=KCTC 32230(T) = JCM 18740(T)).