Purification and gene cloning of a chitosanase from Bacillus ehimensis EAG1

被引：35
|
作者：
Akiyama, K
Fujita, T
Kuroshima, K
Sakane, T
Yokota, A
Takata, R
机构：
[1] Ehime Univ, Fac Agr, Matsuyama, Ehime 7908566, Japan
[2] Takeda Chem Ind Ltd, Div Pharmaceut Res, Yodogawa Ku, Osaka 5328686, Japan
[3] Univ Tokyo, Inst Mol & Cellular Biosci, Bunkyo Ku, Tokyo 1130032, Japan
来源：
JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 1999年 / 87卷 / 03期
关键词：
Bacillus ehimensis; chitosanase; purification; cloning;
D O I：
10.1016/S1389-1723(99)80050-4
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Bacillus ehimensis EAG1 (IFO15659) produced and secreted chitosanase in the presence of exogenous chitosan. The chitosanase was purified from the culture filtrate of the bacterium to apparent homogeneity in SDS-polyacrylamide gel electrophoresis. The purified enzyme had a molecular weight of approximately 31,000, A 1.9-kbp DNA fragment containing the chitosanase gene was cloned and the complete nucleotide sequence was determined. The sequence was found to contain a single open reading frame encoding a protein of 302 amino acids. The deduced amino acid sequence showed significant homology with the chitosanase from Bacillus circulans MH-K1.
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收藏
页码：383 / 385
页数：3
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