The role of adiponectin gene mediated by NF-κB signaling pathway in the pathogenesis of type 2 diabetes

OBJECTIVE: To investigate the role of adiponectin (ADPN) gene mediated by NF-kappa B signaling pathway in the pathogenesis of type 2 diabetes (T2DM). PATIENTS AND METHODS: The levels of expression of ADPN were measured by fluorescence quantitative PCR technology, enzyme-linked immunosorbent assay. Western-blotting and immunohistochemistry in 78 patients with type 2 diabetes. RESULTS: Compared with the normal population, the levels of expression of ADPN and NF-kappa B mRNA in the blood of patients with type 2 diabetes were significantly reduced (p < 0.05). The detection of ADPN gene protein expression in normal population and patients with type 2 diabetes by enzyme-linked immunosorbent assay showed that ADPN gene protein expression in type 2 diabetic patients (1.26 +/- 0.73) mu g/l was significantly lower (p < 0.05) than ADPN gene protein expression of the normal population (3.26 +/- 1.25) mu g/l. At the same time, the detection of the expression of NF-kappa B gene showed that the protein expression in type 2 diabetic patients (0.58 +/- 0.15) mu g/l was significantly lower (p < 0.05) than that in the normal population (1.67 +/- 1.04) mu g/l. The results of Western-blotting were consistent with the results of enzyme-linked immunosorbent assay. lmmunohistochemical results also showed that the percentage of ADPN positive cells in patients with type 2 diabetes (25.47%) was significantly lower (p < 0.05) than that in the normal population (79.47%). CONCLUSIONS: ADPN gene in the human body can be involved in the pathogenesis of T2DM through the NF-kappa B signaling pathway.