The Effect of Lysozyme on Reducing Biofilms by Staphylococcus aureus, Pseudomonas aeruginosa, and Gardnerella vaginalis: An In Vitro Examination

被引:25
|
作者
Hukic, Mirsada [1 ,2 ]
Seljmo, Dzenita [1 ]
Ramovic, Amra [1 ]
Ibrisimovic, Monia Avdic [1 ]
Dogan, Serkan [1 ]
Hukic, Jasna [1 ]
Bojic, Elma Feric [1 ]
机构
[1] Inst Biomed Diagnost & Res Nalaz, Dept Bacteriol & Immunol, Sarajevo, Bosnia & Herceg
[2] Acad Sci & Arts Bosnia & Herzegovina, Dept Med Sci, Sarajevo, Bosnia & Herceg
关键词
lysozyme; biofilm; antibacterial agents; antimicrobial resistance; ANTIBIOTICS; RESISTANCE; BACTERIA; STRAINS; NISIN;
D O I
10.1089/mdr.2016.0303
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Two basic questions about lysozyme activities on the selected microorganisms were investigated, namely whether lysozyme inhibits biofilm production and which concentrations of the enzyme have the ability to change the natural biofilm producing capacity of different strains of Staphylococcus aureus (methicillin sensitive and resistant), Streptococcus pyogenes, Pseudomonas aeruginosa, and Gardnerella vaginalis. The effect of lysozyme on biofilm formation capacities of 16 strains of selected microorganisms was investigated, whereby four testing replicates have been performed in vitro using the Test Tube method, and the potential of lysozyme to change biofilm forming capacities depending on its concentration, species, and strains of microorganisms is demonstrated. A lysozyme concentration of 30g/ml indicated to have the highest inhibiting effect on all tested microorganisms. Furthermore, G. vaginalis was the most sensitive of them all, as its biofilm formation was inhibited in the presence of as low as 2.5g/ml of lysozyme. At enzyme concentrations of 7.5-50g/ml (with the exception of 30g/ml) the biofilm forming capacities of P. aeruginosa were enhanced. Depending on the strain of P. aeruginosa, the total biofilm quantity was either reduced or unaffected at lysozyme concentrations of 2.5, 5, 7.5, and 30g/ml. In contrast, lysozyme concentrations below 15 or 20g/ml did not affect or increase the volume of biofilm formation, while higher concentrations (15, 20, 25g/ml) reduced biofilm formation by 50% (3/6) and 30g/ml of biofilm reduced biofilm forming capacity of S. aureus by 100% (6/6). The results of this study are a strong foundation for further research on lysozyme as a modulator of the biofilm forming capacity of different species with the potential to aid in the development of new drugs for the treatment of oral and vaginal infections.
引用
收藏
页码:353 / 358
页数:6
相关论文
共 50 条
  • [1] Microrheology of Bacterial Biofilms In Vitro: Staphylococcus aureus and Pseudomonas aeruginosa
    Rogers, S. S.
    van der Walle, C.
    Waigh, T. A.
    [J]. LANGMUIR, 2008, 24 (23) : 13549 - 13555
  • [2] Effect of biosurfactants on Pseudomonas aeruginosa and Staphylococcus aureus biofilms in a BioFlux channel
    M. A. Diaz De Rienzo
    P. S. Stevenson
    R. Marchant
    I. M. Banat
    [J]. Applied Microbiology and Biotechnology, 2016, 100 : 5773 - 5779
  • [3] Effect of biosurfactants on Pseudomonas aeruginosa and Staphylococcus aureus biofilms in a BioFlux channel
    De Rienzo, M. A. Diaz
    Stevenson, P. S.
    Marchant, R.
    Banat, I. M.
    [J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2016, 100 (13) : 5773 - 5779
  • [4] Effectiveness of honey on Staphylococcus aureus and Pseudomonas aeruginosa biofilms
    Alandejani, Talal
    Marsan, Joseph
    Ferris, Wendy
    Slinger, Robert
    Chan, Frank
    [J]. OTOLARYNGOLOGY-HEAD AND NECK SURGERY, 2009, 141 (01) : 114 - 118
  • [5] Functionalized polyanilines disrupt Pseudomonas aeruginosa and Staphylococcus aureus biofilms
    Gizdavic-Nikolaidis, Marija R.
    Pagnon, Joanne C.
    Ali, Naseem
    Sum, Reuben
    Davies, Noel
    Roddam, Louise F.
    Ambrose, Mark
    [J]. COLLOIDS AND SURFACES B-BIOINTERFACES, 2015, 136 : 666 - 673
  • [6] Inhibitory Effect of Biocides on the Viable Masses and Matrices of Staphylococcus aureus and Pseudomonas aeruginosa Biofilms
    Tote, K.
    Horemans, T.
    Vanden Berghe, D.
    Maes, L.
    Cos, P.
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2010, 76 (10) : 3135 - 3142
  • [7] Effect of Lactobacillus challenge on Gardnerella vaginalis biofilms
    Saunders, Sheri
    Bocking, Alan
    Challis, John
    Reid, Gregor
    [J]. COLLOIDS AND SURFACES B-BIOINTERFACES, 2007, 55 (02) : 138 - 142
  • [8] DNase Inhibits Gardnerella vaginalis Biofilms In Vitro and In Vivo
    Hymes, Saul R.
    Randis, Tara M.
    Sun, Thomas Yang
    Ratner, Adam J.
    [J]. JOURNAL OF INFECTIOUS DISEASES, 2013, 207 (10): : 1491 - 1497
  • [9] Inhibition of Staphylococcus aureus and Pseudomonas aeruginosa biofilms by quatsomes in low concentrations
    Dong, Dong
    Thomas, Nicky
    Ramezanpour, Mahnaz
    Psaltis, Alkis J.
    Huang, Shuman
    Zhao, Yulin
    Thierry, Benjamin
    Wormald, Peter-John
    Prestidge, Clive A.
    Vreugde, Sarah
    [J]. EXPERIMENTAL BIOLOGY AND MEDICINE, 2020, 245 (01) : 34 - 41
  • [10] Pulsed ultraviolet light inactivation of Pseudomonas aeruginosa and Staphylococcus aureus biofilms
    Garvey, Mary
    Rabbitt, Danny
    Stocca, Alessia
    Rowan, Neil
    [J]. WATER AND ENVIRONMENT JOURNAL, 2015, 29 (01) : 36 - 42