Product Identification and Safety Evaluation of Aflatoxin B1 Decontaminated by Electrolyzed Oxidizing Water

In this study with aflatoxin-contaminated peanuts, the effectiveness of electrolyzed oxidizing water (EOW) in the decontamination of aflatoxin B-1 was investigated. The aflatoxin B-1 content was markedly reduced upon treatment with EOW, particularly with neutral electrolyzed oxidizing water (NEW). The conversion product of EOW treatment was isolated and identified as 8-chloro-9-hydroxy aflatoxin B-1 (compound 1), which is an amphiphilic molecule, in contrast to fat-soluble aflatoxin B-1. A mutagenic response study revealed that the number of revertants per plate after treatment of bacterial strains TA-97, TA-98, TA-100, and TA-102 with NEW was within the standard value range. The HepG2 cell viability assay yielded an IC50 value of compound 1 approximately 150 mM. This study indicates that EOW had the ability to decontaminate aflatoxin B-1, and the conversion product, compound 1, did not exhibit mutagenic activity or cytotoxic effects.