A method for PCR-based identification of bat species from fecal samples

被引:7
|
作者
Korstian, Jennifer M. [1 ]
Schildt, Alison J. [1 ]
Bennett, Victoria J. [2 ]
Williams, Dean A. [1 ]
Hale, Amanda M. [1 ]
机构
[1] Texas Christian Univ, Dept Biol, Ft Worth, TX 76129 USA
[2] Texas Christian Univ, Sch Geol Energy & Environm, Ft Worth, TX 76129 USA
关键词
Bats; Fecal DNA; PCR; Threatened species; Wind energy;
D O I
10.1007/s12686-015-0488-5
中图分类号
X176 [生物多样性保护];
学科分类号
090705 ;
摘要
As bat populations are threatened by a range of factors, there is an increasing need to correctly identify and quantify the cumulative impacts of such factors to individual species. We have developed a fast and reliable method using the polymerase chain reaction to identify bat species from feces found near wind turbines at a north Texas wind farm. The primer sets we developed for the six species found at this site represent an efficient and cost-effective means of obtaining species information from fecal samples. Our approach could readily be applied to other species groups and applications (e.g., degraded carcasses recovered from hibernacula).
引用
收藏
页码:803 / 806
页数:4
相关论文
共 50 条
  • [1] A method for PCR-based identification of bat species from fecal samples
    Jennifer M. Korstian
    Alison J. Schildt
    Victoria J. Bennett
    Dean A. Williams
    Amanda M. Hale
    Conservation Genetics Resources, 2015, 7 : 803 - 806
  • [2] A PCR-BASED IDENTIFICATION METHOD FOR SPECIES OF ARMILLARIA
    HARRINGTON, TC
    WINGFIELD, BD
    MYCOLOGIA, 1995, 87 (02) : 280 - 288
  • [3] A rapid PCR-based test for identification of fifteen British bat species
    Hamilton, P. B.
    Webster, T. M. Uren
    Basiewicz, M.
    Kennedy, E. V.
    De-Bastos, E. S. R.
    Mathews, F.
    CONSERVATION GENETICS RESOURCES, 2015, 7 (03) : 651 - 657
  • [4] A rapid PCR-based test for identification of fifteen British bat species
    P. B. Hamilton
    T. M. Uren Webster
    M. Basiewicz
    E. V. Kennedy
    E. S. R. De-Bastos
    F. Mathews
    Conservation Genetics Resources, 2015, 7 : 651 - 657
  • [5] Detection and species identification of old world Leishmania in clinical samples using a PCR-based method
    Spanakos, Gregory
    Piperaki, Evangelia-Theophano
    Menounos, Panagiotis G.
    Tegos, Nicholas
    Flemetakis, Alexander
    Vakalis, Nicholas C.
    TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 2008, 102 (01) : 46 - 53
  • [6] A PCR-based method for identification of bifidobacteria from the human alimentary tract at the species level
    Venema, K
    Maathuis, AJH
    FEMS MICROBIOLOGY LETTERS, 2003, 224 (01) : 143 - 149
  • [7] A rapid PCR-based method for identification of four important Candida species
    Nazzal, D
    Yasin, S
    Abu-Elteen, K
    NEW MICROBIOLOGICA, 2005, 28 (03): : 245 - 250
  • [8] Investigation of a PCR-based method for the routine identification of British Armillaria species
    Sierra, AP
    Whitehead, DS
    Whitehead, MP
    MYCOLOGICAL RESEARCH, 1999, 103 : 1631 - 1636
  • [9] Rapid species identification of sloth bears from non-invasive samples: a PCR-based assay
    Thatte, Prachi
    Patel, Kaushal
    Ramakrishnan, Uma
    URSUS, 2018, 29 (01) : 67 - 70
  • [10] PCR-Based Identification of Toxinogenic Fusarium Species
    Lacmanova, Iva
    Pazlarova, Jarmila
    Kostelanska, Marta
    Hajslova, Jana
    CZECH JOURNAL OF FOOD SCIENCES, 2009, 27 : 90 - 94