Characterization of polymorphic microsatellite markers for the tideland snail Batillaria flectosiphonata

被引：1
|
作者：
Itoh, Hajime [1 ]
Kamimura, Satomi [2 ]
Hirose, Kimiko [1 ]
Kojima, Shigeaki [1 ]
机构：
[1] Univ Tokyo, Atmosphere & Ocean Res Inst, Kashiwa, Chiba 2778564, Japan
[2] Osaka City Univ, Grad Sch Urban Engn, Sumiyoshi Ku, Sugimoto, Osaka 5588585, Japan
来源：
CONSERVATION GENETICS RESOURCES | 2015年 / 7卷 / 03期
关键词：
Batillaria flectosiphonata; Microsatellite; Next-generation sequencing; PRIMERS; PROGRAM; PCR;
D O I：
10.1007/s12686-015-0452-4
中图分类号：
X176 [生物多样性保护];
学科分类号：
090705 ;
摘要：
Batillaria flectosiphonata is distributed in the Ryukyu Islands, south-western Japan, and shows genetic differentiation among island groups. This species is vulnerable to environmental fluctuations because the connectivity among the groups is low. Therefore, the conservation of each group is necessary to maintain their genetic diversity. Using next-generation sequencing, 10 microsatellite markers were developed for B. flectosiphonata. In Miyara Bay and the Sakashita River estuary, the numbers of alleles per locus were 1-9 and 1-8, and the expected heterozygosities were 0-0.859 and 0-0.655, respectively.
引用
收藏
页码：751 / 753
页数：3
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