Visualizing a Cytostatic Drug and Probing Apoptosis of Cancer Cells

被引：2
|
作者：
Richter, Verena [1 ]
Weber, Petra [1 ]
Wagner, Michael [1 ]
Schickinger, Sarah [1 ]
Bruns, Thomas [1 ]
Schneckenburger, Herbert [1 ]
机构：
[1] Hsch Aalen, Inst Angew Forsch, Beethovenstr 1, D-73430 Aalen, Germany
来源：
CLINICAL AND BIOMEDICAL SPECTROSCOPY AND IMAGING III | 2013年 / 8798卷
关键词：
doxorubicin; 3D cell systems; scattering microscopy; light sheet based fluorescence microscopy; FLIM; FLUORESCENCE LIFETIME; MICROSCOPY; DOXORUBICIN; ADRIAMYCIN;
D O I：
10.1117/12.2032461
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
The uptake and intracellular distribution of the cytostatic drug doxorubicin is visualized in 2D and 3D systems of human breast cancer cells and fibroblasts by fluorescence microscopy and spectroscopy. Fluorescence lifetime imaging (FLIM) and scattering experiments with high angular resolution are suggested to probe apoptotic reactions. A light scattering microscope as well as a light sheet module for 3D fluorescence microscopy have been developed and are used for this purpose.
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页数：5
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