Oceanobacillus chungangensis sp nova, isolated from a sand dune

A Gram-stain-positive, spore-forming, rod-shaped, motile, strictly aerobic bacterial strain, designated CAU 1051(T), was isolated from a sand dune and its taxonomic position was investigated using a polyphasic approach. Strain CAU 1051(T) grew optimally at pH 5.0 and 30 degrees C. NaCl was not required for growth but up to 10.0% (w/v) NaCl was tolerated. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CAU 1051(T) formed a distinct lineage within the genus Oceanobacillus and was most closely related to Oceanobacillus profundus CL-MP28(T), Oceanobacillus caeni S-11(T), and Oceanobacillus picturae LMG 19492(T) (96.8%, 95.6% and 95.3% similarity, respectively). DNA DNA reassociation analysis showed that strain CAU 1051(T) displayed 28.2 +/- 0.7% relatedness to O. profundus KCTC 13625(T). Strain CAU 1051(T) contained MK-7 as the only isoprenoid quinone and anteiso-C-15:0 as the major fatty acid. The cell wall peptidoglycan of strain CAU 1051(T) contained meso-diaminopimelic acid. The polar lipids were composed of diphosphatidylglycerol, phosphatidylglycerol, six unidentified phospholipids, an unidentified glycolipid, and six unidentified polar lipids. The major whole-cell sugars were glucose and ribose. The DNA G+C content was 36.3 mol%. On the basis of phenotypic data and phylogenetic inference, strain CAU 1051(T) represents a novel species of the genus Oceanobacillus for which the name Oceanobacillus chungangensis sp. nov. is proposed. The type strain is CAU 1051(T) (=KCIC 33035(T)=CCUG 63270(T)).