A novel two-enzyme amperometric electrode for lactose determination

被引：14
|
作者：
Gülce, H [1 ]
Gülce, A
Yildiz, A
机构：
[1] Suleyman Demirel Univ, Dept Chem, TR-32260 Isparta, Turkey
[2] Hacettepe Univ, Dept Chem, TR-06532 Ankara, Turkey
来源：
ANALYTICAL SCIENCES | 2002年 / 18卷 / 02期
关键词：
D O I：
10.2116/analsci.18.147
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Coimmobilization of beta-galactosidase and glucose oxidase in a redox polymer, polyvinylferrocenium perchlorate (PVF+ClO4-), led to the development of an enzyme electrode for the determination of lactose. The amperometric response of the electrode was measured at +0.70 V vs. SCE, which was due to the electrooxidation of enzymatically produced H2O2. The effects of the substrate and buffer concentrations as well as the pH on the electrode response were elucidated.
引用
收藏
页码：147 / 150
页数：4
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