Impact of viral attachment factor expression on antibody-mediated neutralization of flaviviruses

被引:4
|
作者
Obara, Christopher J. [1 ,3 ]
Dowd, Kimberly A. [1 ]
Ledgerwood, Julie E. [2 ]
Pierson, Theodore C. [1 ]
机构
[1] NIAID, Viral Pathogenesis Sect, Viral Dis Lab, NIH, Bethesda, MD 20852 USA
[2] NIAID, Vaccine Res Ctr, NIH, Bethesda, MD 20852 USA
[3] Georgetown Univ, Dept Microbiol & Immunol, Washington, DC 20057 USA
关键词
West Nile virus; Dengue virus; Antibody neutralization; Stoichiometric threshold; Attachment factor; WEST-NILE-VIRUS; PROTEIN DOMAIN-III; DENGUE VIRUS; DC-SIGN; STRUCTURAL BASIS; DEPENDENT ENHANCEMENT; DENDRITIC CELLS; INFECTION; ENCEPHALITIS; RECOGNITION;
D O I
10.1016/j.virol.2012.11.016
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Neutralization of flaviviruses requires engagement of the virion by antibodies with a stoichiometry that exceeds a required threshold. Factors that modulate the number of antibodies bound to an individual virion when it contacts target cells impact neutralization potency. However, the contribution of cellular factors to the potency of neutralizing antibodies has not been explored systematically. Here we investigate the relationship between expression level of a viral attachment factor on cells and the neutralizing potency of antibodies. Analysis of the attachment factor DC-SIGNR on cells in neutralization studies failed to identify a correlation between DC-SIGNR expression and antibody-mediated protection. Furthermore, neutralization potency was equivalent on a novel Jurkat cell line induced to express DC-SIGNR at varying levels. Finally, blocking virus-attachment factor interactions had no impact on neutralization activity. Altogether, our studies suggest that cellular attachment factor expression is not a significant contributor to the potency of neutralizing antibodies to flaviviruses. (c) 2012 Elsevier Inc. All rights reserved.
引用
收藏
页码:20 / 27
页数:8
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